Product Pathways - Motif Antibodies
Phospho-Tyrosine Mouse mAb (P-Tyr-102) (Fluorescein Conjugate) #9418
| Applications | Reactivity | Source | Isotype |
|---|---|---|---|
| IF-IC F | All | Mouse | IgG1 |
Applications Key:
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key: All=All species expected
Specificity / Sensitivity
Phospho-Tyrosine Mouse mAb (P-Tyr-102) (Fluorescein Conjugate) is prepared by cross-linking fluorescein to the epsilon amine groups on lysines and amino-terminal amines. Fluorescein absorbs visible blue light at wavelength 490 nm and emits visible green light at 520 nm. This antibody is useful for immunocytochemical detection of tyrosine-phosphorylated proteins. It does not cross-react with proteins phosphorylated on threonine or serine. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Source / Purification
Monoclonal antibody is produced by immunizing mice with phospho-Tyr-containing peptides (KLH coupled). The antibody is purified by protein A chromatography.
Flow Cytometry
Flow cytometric analysis of Phospho-Tyrosine Mouse mAb (P-Tyr-102) (Fluorescein Conjugate) staining of untreated (blue) and pervanadate treated (green) NIH/3T3 cells compared with a nonspecific negative control antibody (red).
IF-IC
Immunocytochemical analysis of 10T1/2 cells, untreated (B) or sodium vanadate-treated (1 mM for 0.5 hour) (D), using Phospho-Tyrosine Mouse mAb (P-Tyr-102) (Fluorescein Conjugate). Images A and C are light micrographs of cells stained in B and D.
Background
Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine Mouse mAbs developed by CST (P-Tyr-100, #9411 and P-Tyr-102, #9416) provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphoyrlation and monitoring tyrosine kinase activity in high throughput drug discovery.
- Schlessinger, J. (2000) Cell 103, 211-225.
- Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-365.
- Ward, S.G. et al. (1992) J. Biol. Chem. 267, 23862-23869.
- Glenney, J.R. et al. (1988) J. Immunol. Methods. 109, 277-285.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9411 Phospho-Tyrosine Mouse mAb (P-Tyr-100)
- 9416 Phospho-Tyrosine Mouse mAb (P-Tyr-102)
- 9417 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated)
- 9419 Immobilized Phospho-Tyrosine Mouse mAb (P-Tyr-100)
- 9414 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 488 Conjugate)
- 9415 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 647 Conjugate)
License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commerical licensing terms please contact CST Business Development at cbunker@cellsignal.com.
