Product Pathways - Motif Antibodies
Immobilized Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Bead Conjugate) #9419
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IP | All | Endogenous | Mouse IgG1 |
Applications Key:
IP=Immunoprecipitation
Reactivity Key: All=All species expected
Protocols
- 9419:
- Immunoprecipitation
Specificity / Sensitivity
Immobilized Phospho-Tyrosine Mouse mAb (P-Tyr-100) is useful for immunoprecipitation of tyrosine-phosphorylated proteins. P-Tyr-100 is immobilized via the conjugation of carbohydrates to cross-linked agarose hydrazide beads. The antibody does not cross-react with proteins phosphorylated on serine or threonine. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Source / Purification
Monoclonal antibody is produced by immunizing animals with phospho-Tyr-containing peptides . Antibody is supplied in Cell Lysis Buffer with 50% glycerol.
IP
Immunoprecipitation of extracts from NIH/3T3 cells, untreated or sodium vanadate-treated (1 mM for 0.5 hour), using Immobilized Phospho-Tyrosine Mouse mAb (P-Tyr-100), followed by Western blot analysis using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411.
Description
Do not use for IAP in the PhosphoScan® method. IAP immobilized antibody is specific to the PhosphoScan® Kit.
Background
Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.
- Schlessinger, J. (2000) Cell 103, 211-25.
- Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
- Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
- Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.
Application References
- Weinberg, J.M. et al. (2001) Am J Pathol 158, 2153-64. Applications: IP
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9411 Phospho-Tyrosine Mouse mAb (P-Tyr-100)
- 9414 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 488 Conjugate)
- 9415 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 647 Conjugate)
- 9416 Phospho-Tyrosine Mouse mAb (P-Tyr-102)
- 9417 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated)
License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Business Development at cbunker@cellsignal.com.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
