Product Pathways - Motif Antibodies
Immobilized Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9419
| Applications | Reactivity | Source | Isotype |
|---|---|---|---|
| IP | All | Mouse | IgG1 |
Applications Key:
IP=Immunoprecipitation
Reactivity Key: All=All species expected
Specificity / Sensitivity
Immobilized Phospho-Tyrosine Mouse mAb (P-Tyr-100) is useful for immunoprecipitation of tyrosine-phosphorylated proteins. P-Tyr-100 is immobilized via the conjugation of carbohydrates to cross-linked agarose hydrazide beads. The antibody does not cross-react with proteins phosphorylated on serine or threonine. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Source / Purification
Monoclonal antibody is produced by immunizing mice with phospho-Tyr-containing peptides (KLH-coupled). Antibody is supplied in Cell Lysis Buffer with 50% glycerol.
IP
Immunoprecipitation of extracts from NIH/3T3 cells, untreated or sodium vanadate-treated (1 mM for 0.5 hour), using Immobilized Phospho-Tyrosine Mouse mAb (P-Tyr-100), followed by Western blot analysis using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411.
Description
Do not use for IAP in the PhosphoScan® method. IAP immobilized antibody is specific to the PhosphoScan® Kit.
Background
Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine Mouse mAbs developed by CST (P-Tyr-100, #9411 and P-Tyr-102, #9416) provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphoyrlation and monitoring tyrosine kinase activity in high throughput drug discovery.
- Schlessinger, J. (2000) Cell 103, 211-225.
- Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-365.
- Ward, S.G. et al. (1992) J. Biol. Chem. 267, 23862-23869.
- Glenney, J.R. et al. (1988) J. Immunol. Methods. 109, 277-285.
Application References
- Weinberg, J. M. et al. (2001) Energetic determinants of tyrosine phosphorylation of focal adhesion proteins during hypoxia/reoxygenation of kidney proximal tubules. Am. J. Pathol. 158, 2153-2164. This article references the use of Immobilized Phospho-Tyrosine Mouse mAb (P-Tyr-100) in the following applications: IP
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9411 Phospho-Tyrosine Mouse mAb (P-Tyr-100)
- 9416 Phospho-Tyrosine Mouse mAb (P-Tyr-102)
- 9417 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated)
- 9418 Phospho-Tyrosine Mouse mAb (P-Tyr-102) (Fluorescein Conjugate)
License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commerical licensing terms please contact CST Business Development at cbunker@cellsignal.com.
