Product Pathways - Metabolism
BCAT2 Antibody #9432
|9432S||100 µl (10 western blots)||---||In Stock||---|
|9432||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
BCAT2 Antibody recognizes endogenous levels of total BCAT2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human BCAT2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing either Myc/DDK-tagged full-length human BCAT1 (hBCAT1-Myc/DDK; +) or Myc/DDK-tagged full-length human BCAT2 (hBCAT2-Myc/DDK; +), using BCAT2 Antibody (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
BCAT1 and BCAT2 are cytosolic and mitochondrial branched chain aminotransferases, respectively (1,2). Research studies have implicated BCAT1 in distant metastasis in patients with advanced colorectal cancer (3). Disruption of BCAT2 in mice leads to higher levels of plasma branched-chain amino acids, reduced adiposity and body weight, and increased energy expenditure, suggesting its role in regulating insulin sensitivity (4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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