Cell Signaling Technology

Product Pathways - Translational Control

Upf1 Antibody #9435

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 140 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Upf1 Antibody detects endogenous levels of total Upf1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Upf1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Upf1 Antibody.

Background

Upf1 was identified as an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons (1). Upf1 was found to be an ATP-dependent RNA helicase in the cytoplasm (2) and was later shown to be a component of cytoplasmic P-bodies (3). Upf1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery (4). Two other active components of NMD, Upf2 and Upf3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively (5).

  1. Leeds, P. et al. (1991) Genes Dev 5, 2303-14.
  2. Weng, Y. et al. (1996) Mol Cell Biol 16, 5477-90.
  3. Bruno, I. and Wilkinson, M.F. (2006) Cell 125, 1036-8.
  4. Isken, O. et al. (2008) Cell 133, 314-27.
  5. Lykke-Andersen, J. et al. (2000) Cell 103, 1121-31.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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