Product Pathways - Translational Control
Upf1 Antibody #9435
|W IP||H M R Mk||Endogenous||140||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Upf1 Antibody detects endogenous levels of total Upf1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Upf1. Antibodies are purified by protein A and peptide affinity chromatography.
Upf1 was identified as an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons (1). Upf1 was found to be an ATP-dependent RNA helicase in the cytoplasm (2) and was later shown to be a component of cytoplasmic P-bodies (3). Upf1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery (4). Two other active components of NMD, Upf2 and Upf3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively (5).
- Leeds, P. et al. (1991) Genes Dev 5, 2303-14.
- Weng, Y. et al. (1996) Mol Cell Biol 16, 5477-90.
- Bruno, I. and Wilkinson, M.F. (2006) Cell 125, 1036-8.
- Isken, O. et al. (2008) Cell 133, 314-27.
- Lykke-Andersen, J. et al. (2000) Cell 103, 1121-31.
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- 9999 Nonfat Dry Milk
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
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- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.