Cell Signaling Technology

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GST (26H1) Mouse mAb (Alexa Fluor® 594 Conjugate) #9440

Applications Reactivity Sensitivity Isotype
IF-IC All Transfected Only Mouse IgG2a

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

GST (26H1) Mouse mAb (Alexa Fluor® 594 Conjugate) detects transfected glutathione S-transferase (GST) fusion proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST fusion protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of transiently transfected COS-7 cells expressing GST-TRCP1 using GST (26H1) Mouse mAb (Alexa Fluor® 594 Conjugate) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Untransfected cells serve as an internal negative control.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated GST (26H1) Mouse mAb #2624.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).

  1. Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7.
  2. Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6.
  3. Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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