Product Pathways -
GST (26H1) Mouse mAb (Alexa Fluor® 594 Conjugate) #9440
|IF-IC||All||Transfected Only||Mouse IgG2a|
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
* Product-specific protocol.
Specificity / Sensitivity
GST (26H1) Mouse mAb (Alexa Fluor® 594 Conjugate) detects transfected glutathione S-transferase (GST) fusion proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a GST fusion protein.
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated GST (26H1) Mouse mAb #2624.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
- Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7.
- Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6.
- Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.
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For Research Use Only. Not For Use In Diagnostic Procedures.