Cell Signaling Technology

Product Pathways - Neuroscience

Nicastrin (D65G7) Rabbit mAb #9447

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 110, 120 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Nicastrin (D65G7) Rabbit mAb recognizes endogenous levels of total nicastrin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala700 of human nicastrin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 and CAD cells using Nicastrin (D65G7) Rabbit mAb.

Background

Nicastrin is a transmembrane glycoprotein serving as an essential component of the γ-secretase complex (1,2). Nicastrin is physically associated with presenilin and plays an important role in the stabilization and correct localization of presenilin to the membrane-bound γ-secretase complex (3). Nicastrin also serves as a docking site for γ-secretase substrates such as APP and Notch, directly binding to them and properly presenting them to γ-secretase to ensure the correct cleavage process (2,4).

  1. Yu, G. et al. (2000) Nature 407, 48-54.
  2. Esler, W. P. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 2720-2725.
  3. Kopan, R. and Goate, A. (2002) Neuron 33, 321-324.
  4. Chen, F. et al. (2001) Nat. Cell Biol. 3, 751-754.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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