Product Pathways - Neuroscience
Nicastrin (D65G7) Rabbit mAb #9447
|W IP||H M R Mk||Endogenous||110, 120||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Nicastrin (D65G7) Rabbit mAb recognizes endogenous levels of total nicastrin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala700 of human nicastrin protein.
Nicastrin is a transmembrane glycoprotein serving as an essential component of the γ-secretase complex (1,2). Nicastrin is physically associated with presenilin and plays an important role in the stabilization and correct localization of presenilin to the membrane-bound γ-secretase complex (3). Nicastrin also serves as a docking site for γ-secretase substrates such as APP and Notch, directly binding to them and properly presenting them to γ-secretase to ensure the correct cleavage process (2,4).
- Yu, G. et al. (2000) Nature 407, 48-54.
- Esler, W. P. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 2720-2725.
- Kopan, R. and Goate, A. (2002) Neuron 33, 321-324.
- Chen, F. et al. (2001) Nat. Cell Biol. 3, 751-754.
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For Research Use Only. Not For Use In Diagnostic Procedures.