Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Ki-67 (8D5) Mouse mAb #9449

Applications Reactivity Sensitivity Isotype
IHC-P IF-IC F H M Endogenous Mouse IgG1

Applications Key:  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Ki-67 (8D5) Mouse mAb recognizes endogenous levels of total Ki-67 protein. Species cross-reactivity for IHC-P is human and mouse, for IF-IC and F, it is human, mouse and rat.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Ki-67 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Ki-67 (8D5) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Ki-67 (8D5) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian serous adenocarcinoma using Ki-67 (8D5) Mouse mAb.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using Ki-67 (8D5) Mouse mAb and Propidium Iodide (PI)/RNase Staining Solution #4087.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Ki-67 (8D5) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).

  1. Gerdes, J. et al. (1983) Int J Cancer 31, 13-20.
  2. Weigel, M.T. and Dowsett, M. (2010) Endocr Relat Cancer 17, R245-62.
  3. Jones, R.L. et al. (2009) Breast Cancer Res Treat 116, 53-68.
  4. Yerushalmi, R. et al. (2010) Lancet Oncol 11, 174-83.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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