Product Pathways - Translational Control
4E-BP1 Antibody #9452
|W IP||H M R Mk||Endogenous||15 to 20||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
4E-BP1 Antibody detects endogenous levels of total 4E-BP1, independent of phosphorylation.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1. Antibodies are purified by protein A affinity chromatography.
Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).
- Pause, A. et al. (1994) Nature 371, 762-767.
- Brunn, G.J. et al. (1997) Science 277, 99-101.
- Gingras, A.C. et al. (1998) Genes Dev. 12, 502-513.
- Fadden, P. et al. (1997) J. Biol. Chem. 272, 10240-10247.
- Gingras, A.C. et al. (1999) Genes Dev. 13, 1422-1437.
- Rapisarda, A. et al. (2004) Topoisomerase I-mediated inhibition of hypoxia-inducible factor 1: mechanism and therapeutic implications. Cancer Res. 64, 1475-1482. Applications: Western Blotting
- Ohne, Y. et al. (2008) J Biol Chem 283, 31861-70. Applications: Western Blotting
- Hara, T. et al. (2008) J Cell Biol 181, 497-510. Applications: Western Blotting
- Wu, M. et al. (2010) Rejuvenation Res 13, 571-9. Applications: Western Blotting
- Fonseca, B.D. et al. (2011) J Biol Chem 286, 27111-22. Applications: Western Blotting
- Fuchs, G. et al. (2011) J Mol Biol 410, 118-30. Applications: Western Blotting
- Ito, N. et al. (2011) Lab Invest 91, 1584-95. Applications: Western Blotting
- Thomson, D.M. et al. (2010) J Appl Physiol 108, 1775-85. Applications: Western Blotting
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- 9644 4E-BP1 (53H11) Rabbit mAb
- 2855 Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
- 9456 Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb
- 9455 Phospho-4E-BP1 (Thr70) Antibody
- 9459 Phospho-4E-BP1 (Thr37/46) Antibody
- 9451 Phospho-4E-BP1 (Ser65) Antibody
- 4923 Non-phospho-4E-BP1 (Thr46) (87D12) Rabbit mAb
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- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.