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4E-BP1 Antibody #9452

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP	H M R Mk	Endogenous	15 to 20	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

9452:

Immunoprecipitation, Western Blotting

Specificity / Sensitivity

4E-BP1 Antibody detects endogenous levels of total 4E-BP1, independent of phosphorylation.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1. Antibodies are purified by protein A affinity chromatography.

Background

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

1. Pause, A. et al. (1994) Nature 371, 762-767.
2. Brunn, G.J. et al. (1997) Science 277, 99-101.
3. Gingras, A.C. et al. (1998) Genes Dev. 12, 502-513.
4. Fadden, P. et al. (1997) J. Biol. Chem. 272, 10240-10247.
5. Gingras, A.C. et al. (1999) Genes Dev. 13, 1422-1437.

Application References

• Rapisarda, A. et al. (2004) Topoisomerase I-mediated inhibition of hypoxia-inducible factor 1: mechanism and therapeutic implications. Cancer Res. 64, 1475-1482. Applications: Western Blotting
• Ohne, Y. et al. (2008) J Biol Chem 283, 31861-70. Applications: Western Blotting
• Hara, T. et al. (2008) J Cell Biol 181, 497-510. Applications: Western Blotting
• Wu, M. et al. (2010) Rejuvenation Res 13, 571-9. Applications: Western Blotting
• Fonseca, B.D. et al. (2011) J Biol Chem 286, 27111-22. Applications: Western Blotting
• Fuchs, G. et al. (2011) J Mol Biol 410, 118-30. Applications: Western Blotting
• Ito, N. et al. (2011) Lab Invest 91, 1584-95. Applications: Western Blotting
• Thomson, D.M. et al. (2010) J Appl Physiol 108, 1775-85. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 9644 4E-BP1 (53H11) Rabbit mAb
• 2855 Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
• 9456 Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb
• 9455 Phospho-4E-BP1 (Thr70) Antibody
• 9459 Phospho-4E-BP1 (Thr37/46) Antibody
• 9451 Phospho-4E-BP1 (Ser65) Antibody
• 4923 Non-phospho-4E-BP1 (Thr46) (87D12) Rabbit mAb
• 9955 4E-BP Antibody Sampler Kit
• 9918 Translational Control Antibody Sampler Kit
• 2845 4E-BP2 Antibody
• 9741 Phospho-eIF4E (Ser209) Antibody
• 9742 eIF4E Antibody
• 7216 PathScan^® Phospho-4E-BP1 (Thr37/Thr46) Sandwich ELISA Kit
• 1053 4E-BP1 Blocking Peptide
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

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For Research Use Only. Not For Use In Diagnostic Procedures.