Product Pathways - Translational Control
Phospho-4E-BP1 (Thr37/46) Antibody #9459
|9459L||300 µl (30 western blots)||---||In Stock||---|
|9459S||100 µl (10 western blots)||---||In Stock||---|
|9459||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||15 to 20||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Phospho-4E-BP1 (Thr37/46) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr37 of mouse 4E-BP1 and Thr46 of mouse 4E-BP1. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293T cells using 4E-BP1 Antibody #9452 (upper) and Phospho-4E-BP1 (Thr37/46) Antibody (lower). The cells were starved for 24 hours in serum-free medium and underwent a 1 hour amino acid deprivation. Amino acids were replenished for 1 hour. Cells were then either untreated (-) or treated with 100 nM insulin (+) for 30 minutes.
Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).
- Jiang, Y. P. et al. (2001) J. Biol. Chem. 276, 10943-10951. Applications: Western Blotting.
- Kwiatkowski, D. J. et al. (2002) Hum. Mol. Genet. 11, 525-534. Applications: Western Blotting.
- Yu, K. et al. (2001) Endocr. Relat. Cancer 8, 249-258. Applications: Western Blotting.
- Ma, D. et al. (2009) J Cell Physiol 219, 31-44. Applications: Western Blotting.
- Ohne, Y. et al. (2008) J Biol Chem 283, 31861-70. Applications: Western Blotting.
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