Product Pathways - PI3K / Akt Signaling
Phospho-FoxO1 (Ser256) Antibody #9461
|W||H M R Mk (C) (Z)||Endogenous||82||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken Z=Zebrafish
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-Fox01 (Ser256) Antibody detects endogenous levels of Fox01 only when phosphorylated at serine 256. The antibody cross-reacts with Fox04 phosphorylated at Ser193.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser256 of human Fox01. Antibodies are purified by protein A and peptide affinity chromatography.
The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).
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- Rena, G. et al. (1999) J. Biol. Chem. 274, 17179-17183.
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- Seoane, J. et al. (2004) Cell 117, 211-223.
- Arden, K.C. (2004) Mol. Cell 14, 416-418.
- Yang, Y. et al. (2005) EMBO J. 24, 1021-1032.
- Camper-Kirby, D. et al. (2001) Myocardial Akt activation and gender: increased nuclear activity in females versus males. Circ. Res. 88, 1020-1027. Applications: Western Blot
- Mochizuki, T. et al. (2002) Akt protein kinase inhibits non-apoptotic programmed cell death induced by ceramide. J. Biol. Chem. 277, 2790-2797. Applications: Western Blotting
- Fan, S. et al. (2001) The multisubstrate adapter Gab1 regulates hepatocyte growth factor (scatter factor)-c-Met signaling for cell survival and DNA repair. Mol. Cell. Biol. 21, 4968-4984. Applications: Western Blot
- Iordanov, M. S. et al. (2002) The UV (ribotoxic) stress response of human keratinocytes involves the unexpected uncoupling of the Ras-extracellular signal-regulated kinase signaling cascade from the activated epidermal growth factor receptor. Mol. Cell. Biol. 22, 5380-5394. Applications: Western Blotting
- Richards, J. S. et al. (2002) Expression of FKHR, FKHRL1, and AFX genes in the rodent ovary: evidence for regulation by IGF-1, estrogen and the gonadotropins. Mol. Endocrin. 16, 580-599. Applications: Western Blotting
- Eke, I. et al. (2010) J Clin Invest 120, 2516-27. Applications: Western Blotting
- Jag, U.R. et al. (2009) Mol Endocrinol 23, 1587-602. Applications: Western Blotting
- Ochi, E. et al. (2010) J Appl Physiol 108, 306-13. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.