Product Pathways - Akt Signaling
FoxO1 Antibody #9462
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W | H | 78 to 82 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
FoxO1 Antibody detects endogenous levels of FoxO1 protein and FoxO4 (at 65-70 kDa). The antibody is sensitive to phosphorylation within the antigenic site and will preferrentially detect FoxO1 (or FoxO4) when not phosphorylated.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human FoxO1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from COS cells, untreated or serum-treated (20%), using Fox01 Antibody.
Background
The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4 and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21CIP1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256 and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).
- Anderson, M.J. et al. (1998) Genomics 47, 187-199.
- Galili, N. et al. (1993) Nat. Genet. 5, 230-235.
- Borkhardt, A. et al. (1997) Oncogene 14, 195-202.
- Nakae, J. et al. (1999) J. Biol. Chem. 274, 15982-15985.
- Rena, G. et al. (1999) J. Biol. Chem. 274, 17179-17183.
- Guo, S. et al. (1999) J. Biol. Chem. 274, 17184-17192.
- Seoane, J. et al. (2004) Cell 117, 211-223.
- Arden, K.C. (2004) Mol. Cell 14, 416-418.
- Yang, Y. et al. (2005) EMBO J. 24, 1021-1032.
Application References
- Fan, S. et al. (2001) The multisubstrate adapter Gab1 regulates hepatocyte growth factor (scatter factor)-c-Met signaling for cell survival and DNA repair. Mol. Cell. Biol. 21, 4968-4984. This article references the use of FoxO1 Antibody in the following applications: Western Blotting
- Richards, J. S. et al. (2002) Expression of FKHR, FKHRL1 and AFX genes in the rodent ovary: evidence for regulation by IGF-1, estrogen, and the gonadotropins. Mol. Endocrin. 16, 580-599. This article references the use of FoxO1 Antibody in the following applications: Western Blotting
- Mochizuki, T. et al. (2002) Akt protein kinase inhibits non-apoptotic programmed cell death induced by ceramide. J. Biol. Chem. 277, 2790-2797. This article references the use of FoxO1 Antibody in the following applications: Western Blotting
- Huang, H. et al. (2004) Androgens negatively regulate forkhead transcription factor FKHR (FOXO1) through a proteolytic mechanism in prostate cancer cells. J. Biol. Chem. 279, 13866-13877. This article references the use of FoxO1 Antibody in the following applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9461 Phospho-FoxO1 (Ser256) Antibody
- 9464 Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody
- 9471 Phospho-FoxO4 (Ser193) Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
