Product Pathways - Motif Antibodies
Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb #9477
|9477S||100 µl (10 western blots)||---||In Stock||---|
|9477||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, E-P=ELISA (Peptide)
Specificity / Sensitivity
Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb recognizes phospho-serine in a (K/H)S*P motif. The antibody does not cross-react with phospho-threonine or phospho-tyrosine containing peptides/proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phospho-CDK substrate peptide library. The antibody is formulated from two rabbit monoclones in order to cover a broad range of reactivity.
Western blot analysis of extracts from COS-7 cells, synchronized in G1/S, G2/M, and G0 phase of the cell cycle, using Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb.
Cyclin-dependent kinases (CDKs) are a family of Ser/Thr kinases that regulate cell-cycle transitions through their association and subsequent phosphorylation of targets in a strictly ordered fashion (1). The substrates for CDKs are proline-directed. The consensus amino acid sequence for CDK substrate is (K/R)(S*)PX(K/R), where X denotes any one of the 20 amino acids (2-4) and S* is the phosphorylation site. Phospho-(Ser) CDKs Substrate Antibody recognizes phosphorylated CDK substrates at their consensus motif, providing a powerful tool for CDK target discovery and characterization, as well as HTS drug screening for potential kinase regulators.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 2325 Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 9998 BSA
- 9999 Nonfat Dry Milk
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
- 7722 Blue Loading Buffer Pack
- 7723 Red Loading Buffer Pack
- 7075 Anti-biotin, HRP-linked Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at firstname.lastname@example.org.