Product Pathways -
Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 594 Conjugate) #9483
PhosphoSitePlus® protein, site, and accession data: Myc
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC | Transfected Only | Mouse IgG2a |
Applications Key:
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9483:
- Immunofluorescence*
* Product-specific protocol.
Specificity / Sensitivity
Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 594 Conjugate) detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).
IF-IC
Confocal immunofluorescent analysis of COS-7 cells, transiently transfected with a construct expressing Myc-tagged chorionic somatomammotropin hormone-like 1 (CSL), using Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 594 Conjugate) (red) and β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 488 Conjugate) #2849 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Untransfected cells provide an internal negative control.
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for immunofluorescent analysis in monkey cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Myc-Tag (9B11) Mouse mAb #2276.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
