Product Pathways - Development

• pathway
• application references
• companion products
• datasheet PDF
• MSDS PDF
• protocols

NDRG1 (D8G9) XP^® Rabbit mAb #9485

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IHC-P IF-IC	H Mk	Endogenous	46, 48	Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

9485:

IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting

Specificity / Sensitivity

NDRG1 (D8G9) XP^® Rabbit mAb recognizes endogenous levels of total NDRG1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NDRG1 protein.

Background

N-myc downstream-regulated gene 1 (NDRG1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG family, which is composed of four members (NDRG1-4) that function in growth, differentiation, and cell survival (1-5). NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage (4), hypoxia (5), and elevated levels of nickel and calcium (2). Expression of NDRG1 is elevated in N-myc defective mice and is negatively regulated by N- and c-myc (1,6). During DNA damage, NDRG1 is induced in a p53-dependent fashion and is necessary for p53-mediated apoptosis (4,7). Research studies have shown that NDRG1 may also play a role in cancer progression by promoting differentiation, inhibiting growth, and modulating metastasis and angiogenesis (3,4,6,8,9). Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (10,11). NDRG1 is up-regulated during mast cell maturation and its deletion leads to attenuated allergic responses (12). Both NDRG1 and NDRG2 are substrates of SGK1, although the precise physiological role of SGK1-mediated phosphorylation is not known (13). NDRG1 is phosphorylated by SGK1 at Thr328, Ser330, Thr346, Thr356, and Thr366. Phosphorylation by SGK1 primes NDRG1 for phosphorylation by GSK-3.

1. Shimono, A. et al. (1999) Mech Dev 83, 39-52.
2. Zhou, D. et al. (1998) Cancer Res 58, 2182-9.
3. van Belzen, N. et al. (1997) Lab Invest 77, 85-92.
4. Kurdistani, S.K. et al. (1998) Cancer Res 58, 4439-44.
5. Park, H. et al. (2000) Biochem Biophys Res Commun 276, 321-8.
6. Li, J. and Kretzner, L. (2003) Mol Cell Biochem 250, 91-105.
7. Stein, S. et al. (2004) J Biol Chem 279, 48930-40.
8. Maruyama, Y. et al. (2006) Cancer Res 66, 6233-42.
9. Nishio, S. et al. (2008) Cancer Lett 264, 36-43.
10. Kalaydjieva, L. et al. (2000) Am J Hum Genet 67, 47-58.
11. Okuda, T. et al. (2004) Mol Cell Biol 24, 3949-56.
12. Taketomi, Y. et al. (2007) J Immunol 178, 7042-53.
13. Murray, J.T. et al. (2004) Biochem J 384, 477-88.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 5196 NDRG1 Antibody
• 9408 NDRG1 (D6C2) Rabbit mAb
• 9395 NDRG1 (D10F8) Rabbit mAb
• 5482 Phospho-NDRG1 (Thr346) (D98G11) XP^® Rabbit mAb
• 3506 Phospho-NDRG1 (Ser330) Antibody
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 8112 SignalStain^® Antibody Diluent
• 8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit)
• 5425 Normal Goat Serum
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)

---

For Research Use Only. Not For Use In Diagnostic Procedures.