Product Pathways - Apoptosis / Autophagy
Cleaved Caspase-7 (Asp198) Antibody #9491
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W IP IC F | H M R | 20 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IC=Immunocytochemistry
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Cleaved Caspase-7 (Asp198) Antibody detects endogenous levels of the large fragment of caspase-7 resulting from cleavage at aspartic acid 198. The antibody does not cross-react with full length caspase-7 or with other caspases.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH coupled) corresponding to amino-terminal residues surrounding Asp198 in human caspase-7. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Jurkat cells and 293 cells, untreated, cytochrome c-treated (0.25 mg/ml, 1 hour) or etoposide-treated (25 µM, 5 hours), using Cleaved Caspase-7 (Asp198) Antibody.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Cleaved Caspase-7 (Asp198) Antibody compared to a nonspecific negative control antibody (red).
IF-IC
Immunostaining of cerebellar neuron culture from newborn rat, untreated or tert-Butyl-hydroperoxide-treated (tBH, a free radical, 40 µM for 30 minutes folowed by 3 hour recovery), showing colocalization of cleaved caspase-7 with apoptotic neurons, using Cleaved Caspase-7 (Asp198) Antibody (green) and propidium iodide (red).
Background
Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (2-4). Caspase-7 is an effector caspase (along with caspase-2 and -3), meaning that it cleaves essential cellular machinery rather than activating other caspases (5-8). Caspase-7 is cleaved by many enzymes, including caspases-3, -6, -8, -9 and granzyme B (1,4,5). Once activated, caspase-7 cleaves many of the same substrates as caspase-3, including poly (ADP-ribose) polymerase, or PARP (2,4).
- Fernandes-Alnemri, T. et al. (1995) Cancer Res. 55, 6045-6052.
- Duan, H. et al. (1996) J. Biol. Chem. 271, 1621-1625.
- Lippke, J. A. et al. (1996) J. Biol. Chem. 271, 1825-1828.
- Cohen, G.M. (1997) Biochem. J. 326, 1-16.
- Thornberry, N. A. et al. (1997) J. Biol. Chem. 272, 17907-17911.
- Chandler, J. M. et al. (1998) J. Biol. Chem. 273, 10815-10818.
- MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.
- Nu?ez, G. et al. (1998) Oncogene 17, 3237-3245.
Application References
- Guegan, C. et al. (2001) Recruitment of the mitochondrial-dependent apoptotic pathway in amyotrophic lateral sclerosis. J. Neurosci. 21, 6569-6576. This article references the use of Cleaved Caspase-7 (Asp198) Antibody in the following applications: IHC-FL (floating/frozen)
- Han, H. et al. (2001) Oxidative preconditioning and apoptosis in L-cells. Roles of protein kinase B and mitogen-activated protein kinases. J. Biol. Chem. 276 (28), 26357-26364. This article references the use of Cleaved Caspase-7 (Asp198) Antibody in the following applications: Western Blotting
- Wang, J. et al. (2001) Increased in vivo apoptosis in cells lacking mitochondrial DNA gene expression. Proc. Natl. Acad. Sci. USA 98 (7), 4038-4043. This article references the use of Cleaved Caspase-7 (Asp198) Antibody in the following applications: IHC-FL (floating/frozen)
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