Cell Signaling Technology

Product Pathways - Apoptosis

Caspase-7 (C7) Mouse mAb (Human Specific) #9494

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Endogenous 20, 30, 35 Mouse IgG1

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Caspase-7 (C7) Mouse mAb (Human Specific) detects endogenous levels of caspase-7 proform as well as the 30 and 20 kDa cleaved fragments. This antibody does not cross-react with other caspases.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human caspase-7 protein.

Western Blotting

Western Blotting

Western blot analysis of extract from Jurkat cells, untreated or etoposide-treated, using Caspase-7 (C7) Mouse mAb (Human Specific).

Background

Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP (1,3). During apoptosis, caspase-7 is activated through proteolytic processsing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits (1,3). Similar to caspase-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD (5).

  1. Fernandes-Alnemri, T. et al. (1995) Cancer Res. 55, 6045-6052.
  2. Duan, H. et al. (1996) J. Biol. Chem. 271, 1621-1625.
  3. Lippke, J. A. et al. (1996) J. Biol. Chem. 271, 1825-1828.
  4. Cohen, G.M. (1997) Biochem. J. 326, 1-16.
  5. Thornberry, N. A. et al. (1997) J. Biol. Chem. 272, 17907-17911.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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