Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

Caspase-7 (C7) Mouse mAb (Human Specific) #9494

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Endogenous 20, 30, 35 Mouse IgG1

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Caspase-7 (C7) Mouse mAb (Human Specific) detects endogenous levels of caspase-7 proform as well as the 30 and 20 kDa cleaved fragments. This antibody does not cross-react with other caspases.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human caspase-7 protein.

Western Blotting

Western Blotting

Western blot analysis of extract from Jurkat cells, untreated or etoposide-treated, using Caspase-7 (C7) Mouse mAb (Human Specific).

Background

Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (2-4). Caspase-7 is an effector caspase (along with caspase-2 and -3), meaning that it cleaves essential cellular machinery rather than activating other caspases (5-8). Caspase-7 is cleaved by many enzymes, including caspases-3, -6, -8, -9 and granzyme B (1,4,5). Once activated, caspase-7 cleaves many of the same substrates as caspase-3, including poly (ADP-ribose) polymerase, or PARP (2,4).

  1. Fernandes-Alnemri, T. et al. (1995) Cancer Res. 55, 6045-6052.
  2. Duan, H. et al. (1996) J. Biol. Chem. 271, 1621-1625.
  3. Lippke, J. A. et al. (1996) J. Biol. Chem. 271, 1825-1828.
  4. Cohen, G.M. (1997) Biochem. J. 326, 1-16.
  5. Thornberry, N. A. et al. (1997) J. Biol. Chem. 272, 17907-17911.
  6. Chandler, J. M. et al. (1998) J. Biol. Chem. 273, 10815-10818.
  7. MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.
  8. Nu–ez, G. et al. (1998) Oncogene 17, 3237-3245.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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