Cell Signaling Technology

Product Pathways - Stem Cell and Lineage Markers

Nucleostemin Antibody #9495

Applications Reactivity Sensitivity MW (kDa) Source
W H (M) (Mk) Endogenous 65 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Nucleostemin Antibody recognizes endogenous levels of total nucleostemin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human nucleostemin protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Nucleostemin Antibody.

Background

Nucleostemin (GNL3) is a member of the MMR1/HSR1 GTP-binding protein family. It is essential in early embryogenesis (1) and investigators have shown that nucleostemin participates in the control of stem and cancer cell cycle proliferation (2), possibly through regulation of p53 activity (3). Nucleostemin has been found to be expressed in CNS stem cells, embryonic stem cells, and several cancer cell lines, and is localized to both the nucleus and the nucleolus in a cell-cycle dependent manner (4).

  1. Beekman, C. et al. (2006) Mol Cell Biol 26, 9291-301.
  2. Ivanova, N.B. et al. (2002) Science 298, 601-4.
  3. Dai, M.S. et al. (2008) Mol Cell Biol 28, 4365-76.
  4. Tsai, R.Y. and McKay, R.D. (2002) Genes Dev 16, 2991-3003.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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