Product Pathways - Apoptosis / Autophagy
Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb #9496
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H | Endogenous | 18, 41, 43 | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb detects human Caspase-8 when cleaved at Asp391 (isoform A, Asp374 on isoform B). This antibody will detect cleavage products containing the pro-domain with the p18 subunit as well as the p18 subunit alone.
Source / Purification
Rabbit monoclonal antibodies are produced from spleens taken from rabbits immunized with a synthetic peptide (KLH-coupled) corresponding to residues adjacent to Asp391 of human Caspase-8.
Western Blotting
Western blot analysis of HeLa cells transfected with the death receptors Fas and DR5, or treated with cycloheximide (CHX) and TNF-alpha using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb (upper), or total Caspase-8 (1C12) Mouse mAb (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon (chronic inflammation) using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HeLa cells, untreated (left) or Cycloheximide and TNFα-treated (right), using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb compared to a nonspecific negative control antibody (red).
IF-IC
Immunofluorescent staining of HeLa cells untreated or treated with cytotoxic anti-Fas Antibody, using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb.
Background
Apoptosis induced through CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6 and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.
- Muzio, M. et al. (1996) Cell 85, 817-827.
- Boldin, M.P. et al. (1996) Cell 85, 803-815.
- Fernandes-Alnemri, T. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 7464-7469.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
