Product Pathways - Apoptosis
Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb #9496
PhosphoSitePlus® protein, site, and accession data: Casp8
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H | Endogenous | 18, 41, 43 | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb detects human Caspase-8 when cleaved at Asp391 (isoform A, Asp374 on isoform B). This antibody will detect cleavage products containing the pro-domain with the p18 subunit as well as the p18 subunit alone.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to Asp391 of human Caspase-8.
Western Blotting
Western blot analysis of HeLa cells transfected with the death receptors Fas and DR5, or treated with cycloheximide (CHX) and TNF-alpha using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb (upper), or total Caspase-8 (1C12) Mouse mAb (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon (chronic inflammation) using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HeLa cells, untreated (left) or Cycloheximide and TNFα-treated (right), using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb compared to a nonspecific negative control antibody (red).
IF-IC
Immunofluorescent staining of HeLa cells untreated or treated with cytotoxic anti-Fas Antibody, using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb.
Background
Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.
- Muzio, M. et al. (1996) Cell 85, 817-827.
- Boldin, M.P. et al. (1996) Cell 85, 803-815.
- Fernandes-Alnemri, T. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 7464-7469.
Application References
- Biton, S. and Ashkenazi, A. (2011) Cell 145, 92-103. Applications: Western Blotting
- D'Agostino, L. and Giordano, A. (2011) Oncotarget 2, 1055-74. Applications: Western Blotting
- Jane, E.P. et al. (2011) Mol Cancer Ther 10, 198-208. Applications: Western Blotting
- Jinesh G, G. et al. (2012) J Leukoc Biol , . Applications: Western Blotting
- Thome, C.H. et al. (2012) Mol Cell Proteomics , . Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9746 Caspase-8 (1C12) Mouse mAb
- 9748 Cleaved Caspase-8 (Asp384) (11G10) Mouse mAb
- 4927 Caspase-8 Antibody (Mouse Specific)
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 1011 Cleaved Caspase-8 (Asp391) Blocking Peptide
- 8112 SignalStain® Antibody Diluent
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.
