Product Pathways - Apoptosis / Autophagy
Caspase-9 Antibody (Human Specific) #9502
PhosphoSitePlus® protein, site, and accession data: Casp9
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W F | H | Endogenous | 35, 37, 47 | Rabbit |
Applications Key:
W=Western Blotting
F=Flow Cytometry
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9502:
- Flow, Western Blotting
Specificity / Sensitivity
Caspase-9 Antibody detects endogenous levels of full length caspase-9 (47 kDa) and large fragments of caspase-9. The antibody does not recognize other caspases.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding aspartic acid 315 of human caspase-9. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of HeLa cells, untreated, staurosporine-treated (1 µM) or cytochrome c-treated (0.25 mg/ml), and Jurkat cells, untreated or cytochrome c-treated, using Caspase-9 Antibody (Human Specific).
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Caspase-9 Antibody (Human Specific) compared to a nonspecific negative control antibody (red).
Background
Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).
- Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724.
- Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106.
- Liu, X. et al. (1996) Cell 86, 147-157.
- Li, P. et al. (1997) Cell 91, 479-489.
- Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556.
- Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57.
- Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
- Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292.
- Sun, X. et al. (1999) J. Biol. Chem. 274, 5053-5060.
- MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.
Application References
- Jiang, C. et al. (2001) Caspases as key executors of methyl selenium-induced apoptosis (anoikis) of DU-145 prostate cancer cells. Cancer Res. 61, 3062-3070. Applications: Western Blotting
- Yu, W. et al. (2003) RRR-alpha-tocopheryl succinate-induced apoptosis of human breast cancer cells involves Bax translocation to mitochondria. Cancer Res. 63, 2483-2491. Applications: Western Blotting
- Jeong, W. et al. (2004) Roles of TRP14, a thioredoxin-related protein in tumor necrosis factor-alpha signaling pathways. J. Biol Chem. 279 (5), 3151-9. Applications: Western Blotting
- Bhakar, A. L. et al. (2003) Apoptosis induced by p75NTR overexpression requires Jun kinase-dependent phosphorylation of Bad. J. Neurosci. 23, 11373-11381. Applications: Western Blotting
- Chandrasekar, B. et al. (2004) Activation of intrinsic and extrinsic proapoptotic signaling pathways in interleukin-18-mediated human cardiac endothelial cell death. J. Biol. Chem. 279, 20221-20233. Applications: Western Blotting
- Sun, S. Y. et al. (2002) The synthetic retinoid CD437 selectively induces apoptosis in human lung cancer cells while sparing normal human lung epithelial cells. Cancer Res. 62, 2430-2436. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9501 Cleaved Caspase-9 (Asp330) Antibody (Human Specific)
- 9504 Caspase-9 Antibody (Mouse Specific)
- 9505 Cleaved Caspase-9 (Asp315) Antibody (Human Specific)
- 9852 Chaps Cell Extract Buffer (10X)
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
