Product Pathways - Apoptosis / Autophagy
Caspase-9 Antibody (Rat Specific) #9506
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W | R | 17, 38, 40, 51 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Caspase-9 Antibody (Rat Specific) detects endogenous levels of full length (51 kDa) and large fragments of caspase-9. The antibody does not recognize any other cleaved caspases.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding aspartic acid 353 of rat caspase-9. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from PC12 cells, untreated or staurosporine-treated (3 µM), using Caspase-9 Antibody (Rat Specific). 51 kDa (full length); 40 kDa (prodomain + large fragment cleaved at Asp368); 38 kDa (prodomain + large fragment cleaved at Asp353); 17 kDa (large fragment)
Background
Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. This complex processes procaspase-9 into a large active fragment (35 kDa or 17 kDa) and a small fragment (10 kDa) by self-cleavage at Asp315 (3-5). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (6-9). In addition to self-cleavage, procaspase-9 can also be cleaved in vivo by caspase-3 at Asp330. This process serves as positive feedback to amplify the apoptotic signal in the caspase activation pathway (3-5).
- Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724.
- Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106.
- Liu, X. et al. (1996) Cell 86, 147-157.
- Li, P. et al. (1997) Cell 91, 479-489.
- Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556.
- Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
- Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292.
- Sun, X. et al. (1999) J. Biol. Chem. 274, 5053-5060.
- MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.
Application References
- Marques, C. A. et al. (2003) Neurotoxic mechanisms caused by the Alzheimer's disease-linked Swedish amyloid precursor protein mutation: oxidative stress, caspases, and the JNK pathway. J. Biol. Chem. 278, 28294-28302. This article references the use of Caspase-9 Antibody (Rat Specific) in the following applications: Western Blotting
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Companion Products
- 9501 Cleaved Caspase-9 (Asp330) Antibody (Human Specific)
- 9502 Caspase-9 Antibody (Human Specific)
- 9504 Caspase-9 Antibody (Mouse Specific)
- 9505 Cleaved Caspase-9 (Asp315) Antibody (Human Specific)
- 9507 Cleaved Caspase-9 (Asp353) Antibody (Rat Specific)
- 9852 Chaps Cell Extract Buffer (10X)
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
