Product Pathways - Cell Cycle / Checkpoint
Phospho-cdc25C (Ser216) Antibody #9528
PhosphoSitePlus® protein, site, and accession data: Cdc25C
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Endogenous | 60 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9528:
- Western Blotting
Specificity / Sensitivity
Phospho-cdc25C (Ser216) Antibody detects endogenous levels of cdc25C only when phosphorylated at Ser216.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser216 of human cdc25C. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of wild type GST-cdc25C and mutant GST-cdc25C Ser216Ala fusion proteins, untreated or phosphorylated by Chk2, using Phospho-cdc25C (Ser216) Antibody.
Western Blotting
Western blot analysis of GST-cdc25C phosphorylated by cdc2/cyclin B (New England Biolabs #P6020), Chk2 or both kinases, using Phospho-cdc25C (Ser216) Antibody (upper) or Phospho-cdc25C (Ser214) antibody (lower).
Western Blotting
Western Blot analysis of HT29 cells, untreated, nocodazole-treated, and lambda phosphotase-treated, using Phospho-cdc25C (Ser216) antibody (upper), and cdc25C (5H9) Rabbit mAb, #4688, (lower).
Background
cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).
- Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.
- Peng, C.Y. et al. (1997) Science 277, 1501-1505.
- Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.
- Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.
- Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.
Application References
- Tan, M. et al. (2002) Phosphorylation on tyrosine-15 of p34(Cdc2) by ErbB2 inhibits p34(Cdc2) activation and is involved in resistance to taxol-induced apoptosis. Mol. Cell 9, 993-1004. Applications: Western Blotting
- Bulavin, D. V. et al. (2001) Initiation of a G2/M checkpoint after ultraviolet radiation requires p38 kinase. Nature 411, 102-107. Applications: Western Blotting
- Hirose, Y. et al. (2001) Abrogation of the Chk1-mediated G(2) checkpoint pathway potentiates temozolomide-induced toxicity in a p53-independent manner in human glioblastoma cells. Cancer Res. 61, 5843-5849. Applications: Western Blotting
- Margolis, S. S. et al. (2003) PP1 control of M phase entry exerted through 14-3-3-regulated Cdc25 dephosphorylation. EMBO J. 22, 5734-5745. Applications: Western Blotting
- Harvey, S. et al. (2001) Interactions between 2-fluoroadenine 9-beta-D-arabinofuranoside and the kinase inhibitor UCN-01 in human leukemia and lymphoma cells. Clin. Cancer Res. 7, 320-330. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2341 Phospho-Chk1 (Ser345) Antibody
- 2661 Phospho-Chk2 (Thr68) Antibody
- 9527 Phospho-cdc25C (Thr48) Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 4901 Phospho-cdc25C (Ser216) (63F9) Rabbit mAb
- 4688 cdc25C (5H9) Rabbit mAb
- 9529 Phospho-cdc25C (Ser198) Antibody
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
