Product Pathways - Cell Cycle / Checkpoint

Phospho-cdc25C (Ser216) Antibody #9528

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Applications	Reactivity	Sensitivity	MW (kDa)	Source
W	H	Endogenous	60	Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-cdc25C (Ser216) Antibody detects endogenous levels of cdc25C only when phosphorylated at Ser216.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser216 of human cdc25C. Antibodies are purified by protein A and peptide affinity chromatography.

Background

cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).

1. Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.
2. Peng, C.Y. et al. (1997) Science 277, 1501-1505.
3. Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.
4. Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.
5. Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.

Application References

• Tan, M. et al. (2002) Phosphorylation on tyrosine-15 of p34(Cdc2) by ErbB2 inhibits p34(Cdc2) activation and is involved in resistance to taxol-induced apoptosis. Mol. Cell 9, 993-1004. This article references the use of Phospho-cdc25C (Ser216) Antibody in the following applications: Western Blotting
• Bulavin, D. V. et al. (2001) Initiation of a G2/M checkpoint after ultraviolet radiation requires p38 kinase. Nature 411, 102-107. This article references the use of Phospho-cdc25C (Ser216) Antibody in the following applications: Western Blotting
• Hirose, Y. et al. (2001) Abrogation of the Chk1-mediated G(2) checkpoint pathway potentiates temozolomide-induced toxicity in a p53-independent manner in human glioblastoma cells. Cancer Res. 61, 5843-5849. This article references the use of Phospho-cdc25C (Ser216) Antibody in the following applications: Western Blotting
• Margolis, S. S. et al. (2003) PP1 control of M phase entry exerted through 14-3-3-regulated Cdc25 dephosphorylation. EMBO J. 22, 5734-5745. This article references the use of Phospho-cdc25C (Ser216) Antibody in the following applications: Western Blotting
• Harvey, S. et al. (2001) Interactions between 2-fluoroadenine 9-beta-D-arabinofuranoside and the kinase inhibitor UCN-01 in human leukemia and lymphoma cells. Clin. Cancer Res. 7, 320-330. This article references the use of Phospho-cdc25C (Ser216) Antibody in the following applications: Western Blotting

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.