Product Pathways - Apoptosis / Autophagy
PARP (46D11) Rabbit mAb #9532
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-IC | H M R Mk | Endogenous | 116, 89 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
PARP (46D11) Rabbit mAb detects endogenous levels of total full-length PARP and the large fragment (89 kDa) produced by caspase cleavage.
Source / Purification
Rabbit monoclonal antibodies are prepared from rabbits immunized with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Gly623 of PARP.
Western Blotting
Western blot analysis of extracts from THP-1 cells with or without treatment with TNF-α and cycloheximide as well as control extracts from SW620 and A20 cell lines, using PARP (46D11) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of untreated HeLa cells labeled with PARP (46D11) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
Background
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
- Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
- Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
- Cohen, G.M. (1997) Biochem. J. 326, 1-16.
- Nicholson, D. W. et al. (1995) Nature 376, 37-43.
- Tewari, M. et al. (1995) Cell 81, 801-809.
- Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9546 Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific)
- 9548 Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific)
- 9541 Cleaved PARP (Asp214) Antibody (Human Specific)
- 9544 Cleaved PARP (Asp214) Antibody (Mouse Specific)
- 9547 Cleaved PARP (Asp214) Antibody (Human Specific) (Fluorescein Conjugate)
- 9545 Cleaved PARP (Asp214) Antibody (Rat Specific)
- 9542 PARP Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
