Product Pathways - Lymphocyte Signaling
NTAL/LAB Antibody #9533
PhosphoSitePlus® protein, site, and accession data: LAB
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H | Endogenous | 25 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
NTAL/LAB Antibody detects endogenous levels of total NTAL/LAB protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly222 of human NTAL/LAB. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of total cell lysates from SR and SEM cells, using NTAL/LAB Antibody.
Background
Non-T cell activation linker (NTAL)/linker for activation of B cells (LAB) is a small transmembrane adaptor protein associated with glycolipid-enriched membrane fractions (1,2). NTAL/LAB is also known as LAT2 (linker for activation of T cells 2), WBSCR5, WBS15, and WBSCR15 (Williams-Beuren syndrome chromosome region 15 protein). It is expressed in B cells, monocytes, mast cells, and natural killer cells, but not in resting T cells (3). Upon activation of several receptors, NTAL/LAB becomes tyrosine-phosphorylated and recruits signaling molecules such as GRB2 and c-Cbl into receptor signaling complexes (4-6).
- Iwaki, S. et al. (2007) Int J Biochem Cell Biol 39, 868-73.
- Lindquist, J.A. et al. (2003) Immunol. Rev. 191, 165-182.
- Brdicka, T. et al. (2002) J. Exp. Med. 196, 1617-1626.
- Koonpaew, S. et al. (2004) J. Biol. Chem. 279, 11229-11235.
- Tkaczyk, C. et al. (2004) Blood 104, 207-214.
- Stork, B. et al. (2004) Immunity 21, 681-691.
Application References
- Thome, C.H. et al. (2012) Mol Cell Proteomics , . Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
