Product Pathways - Apoptosis
PDCD4 (D29C6) XP® Rabbit mAb #9535
PhosphoSitePlus® protein, site, and accession data: PDCD4
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC | H M R Mk | Endogenous | 60 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
PDCD4 (D29C6) XP® Rabbit mAb detects endogenous levels of total PDCD4 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to human PDCD4 protein.
Western Blotting
Western blot analysis of extracts from various cell types using PDCD4 (D29C6) XP® Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PDCD4 (D29C6) XP® Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human tonsil using PDCD4 (D29C6) XP® Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or serum-starved (right), using PDCD4 (D29C6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Background
PDCD4 (programmed cell death protein 4) was identified as a suppressor of neoplastic transformation (1). It interacts with eukaryotic initiation factors eIF4AI and eIF4AII (2). PDCD4 colocalizes with eIF4A in the cytoplasm and inhibits the activity of eIF4A as an ATP-dependent RNA helicase (2). PDCD4 inhibits cap-dependent translation (2). Upon stimulation by mitogens, PDCD4 is phosphorylated on Ser67 by S6K1 kinase and then degraded through the ubiquitin pathway leading to enhanced translation and cell growth (3).
- Cmarik, J.L. et al. (1999) Proc Natl Acad Sci USA 96, 14037-42.
- Yang, H.S. et al. (2003) Mol Cell Biol 23, 26-37.
- Dorrello, N.V. et al. (2006) Science 314, 467-71.
Application References
- Yen, W.C. et al. (2012) Clin Cancer Res 18, 5374-86. Applications: IHC-P (paraffin)
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For Research Use Only. Not For Use In Diagnostic Procedures.
