Cell Signaling Technology

Product Pathways - Glucose Metabolism

Enolase-2 Antibody #9536

Applications Reactivity Sensitivity MW (kDa) Source
W H M Endogenous 47 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Enolase-2 Antibody detects endogenous levels of total enolase-2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human enolase-2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and human and mouse brain using Enolase-2 Antibody.

Background

Enolase is a glycolytic enzyme that is involved in the conversion of 2-phosphoglycerate to phosphoenolpyruvate (1). Mammalian enolase has three subunits: α, β, and γ, that can form homo and heterodimers. Homodimers of γ enolase are neuronal-specific (2). Clinical studies have shown elevated levels of neuro-specific enolase-2 in neuroblastoma (2) and small-cell lung cancer (3,4). Hence, enolase-2 is widely used as a diagnostic marker in a variety of clinical assays (3,4).

  1. Van Obberghen, E. et al. (1988) J Neurosci Res 19, 450-6.
  2. Pancholi, V. (2001) Cell Mol Life Sci 58, 902-20.
  3. Stern, P. et al. (2007) Tumour Biol 28, 84-92.
  4. O'Shea, P. et al. (1995) Ir J Med Sci 164, 31-6.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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