Cell Signaling Technology

Product Pathways - Metabolism

Enolase-2 Antibody #9536

Applications Reactivity Sensitivity MW (kDa) Source
W H M Endogenous 47 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Enolase-2 Antibody detects endogenous levels of total enolase-2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human enolase-2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and human and mouse brain using Enolase-2 Antibody.

Background

Enolase is a glycolytic enzyme that is involved in the conversion of 2-phosphoglycerate to phosphoenolpyruvate (1). Mammalian enolase has three subunits: α, β, and γ, that can form homo and heterodimers. Homodimers of γ enolase are neuronal-specific (2). Research studies have shown elevated levels of neuro-specific enolase-2 in neuroblastoma (2) and small-cell lung cancer (3,4).

  1. Van Obberghen, E. et al. (1988) J Neurosci Res 19, 450-6.
  2. Pancholi, V. (2001) Cell Mol Life Sci 58, 902-20.
  3. Stern, P. et al. (2007) Tumour Biol 28, 84-92.
  4. O'Shea, P. et al. (1995) Ir J Med Sci 164, 31-6.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products