Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Translational Control

Ribosomal Protein S3 (D50G7) XP® Rabbit mAb #9538

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Mk Endogenous 27 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Ribosomal Protein S3 (D50G7) XP® Rabbit mAb detects endogenous levels of total ribosomal protein S3.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ribosomal protein S3.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Ribosomal Protein S3 (D50G7) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of C6 cells using Ribosomal Protein S3 (D50G7) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Ribosomal protein S3 (rpS3) is a component of the 40S ribosomal subunit and is involved in translation. HSP90 interacts with both the amino-terminus and carboxy-terminus of rpS3, preventing its ubiquitination and degradation and thereby retaining the integrity of the ribosome (1). rpS3 has also been shown to function as an endonuclease during DNA damage repair (2,3). Furthermore, overexpression of rpS3 sensitizes lymphocytic cells to cytokine-induced apoptosis, indicating a third role for rpS3 during apoptosis (4). The functions of rpS3 during DNA damage repair and apoptosis have been mapped to two distinct domains (4).

  1. Kim, T.S. et al. (2006) Mol Biol Cell 17, 824-33.
  2. Kim, J. et al. (1995) J Biol Chem 270, 13620-9.
  3. Choi, S.H. et al. (2006) FEBS Lett 580, 6755-62.
  4. Jang, C.Y. et al. (2004) FEBS Lett 560, 81-5.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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