Product Pathways - Apoptosis / Autophagy

Cleaved PARP (Asp214) Antibody (Human Specific) #9541

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Applications	Reactivity	MW (kDa)	Source
W IHC-P IF-IC IC F	H	89	Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  IC=Immunocytochemistry  F=Flow Cytometry
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Cleaved PARP (Asp214) Antibody (Human Specific) detects endogenous levels of the large fragment (89 kDa) of human PARP1 produced by caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to carboxy-terminal residues surrounding Asp214 in human PARP. Antibodies are purified by protein A and peptide affinity chromatography.

Background

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)

1. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
2. Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
3. Cohen, G.M. (1997) Biochem. J. 326, 1-16.
4. Nicholson, D. W. et al. (1995) Nature 376, 37-43.
5. Tewari, M. et al. (1995) Cell 81, 801-809.
6. Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.

Application References

• Brunet, A. et al. (2001) Protein kinase SGK mediates survival signals by phosphorylating the Forkhead transcription factor FKHRL1 (FOXO3a). Mol. Cell. Biol. 21, 952-965. This article references the use of Cleaved PARP (Asp214) Antibody (Human Specific) in the following applications: Western Blotting
• Bhakar, A. L. et al. (2003) Apoptosis induced by p75NTR overexpression requires Jun kinase-dependent phosphorylation of Bad. J. Neurosci. 23, 11373-11381. This article references the use of Cleaved PARP (Asp214) Antibody (Human Specific) in the following applications: Western Blotting
• Jiang, C. et al. (2001) Caspases as key executors of methyl selenium-induced apoptosis (anoikis) of DU-145 prostate cancer cells. Cancer Res. 61, 3062-3070. This article references the use of Cleaved PARP (Asp214) Antibody (Human Specific) in the following applications: Western Blotting

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