Product Pathways - Apoptosis
Cleaved PARP (Asp214) Antibody (Mouse Specific) #9544
PhosphoSitePlus® protein, site, and accession data: PARP1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | M | Endogenous | 89 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Cleaved PARP (Asp214) Antibody (Mouse Specific) detects endogenous levels of the large fragment (89 kDa) of mouse PARP1 resulting from caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues surrounding Asp214 in mouse PARP. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of NIH/3T3 cells, untreated or staurosporine-treated (1 µM, 3hrs), using Cleaved PARP (Asp214) Antibody (Mouse Specific).
IF-IC
Confocal immunofluorescent analysis of 3T3-L1 cells, untreated (left) or staurosporine-treated (right), using Cleaved PARP (Asp214) Antibody (Mouse Specific) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)
- Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
- Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
- Cohen, G.M. (1997) Biochem. J. 326, 1-16.
- Nicholson, D. W. et al. (1995) Nature 376, 37-43.
- Tewari, M. et al. (1995) Cell 81, 801-809.
- Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.
Application References
- Garnier, P. et al. (2003) Ischemic preconditioning by caspase cleavage of poly(ADP-ribose) polymerase-1. J. Neurosci. 23 (22), 7967-73. Applications: IC-IF Western Blotting
- Benedettini, E. et al. (2010) Am J Pathol 177, 415-23. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9548 Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific)
- 9542 PARP Antibody
- 9541 Cleaved PARP (Asp214) Antibody (Human Specific)
- 9545 Cleaved PARP (Asp214) Antibody (Rat Specific)
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- 9665 Caspase-3 (8G10) Rabbit mAb
- 9491 Cleaved Caspase-7 (Asp198) Antibody
- 9492 Caspase-7 Antibody
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- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
