Product Pathways - Apoptosis
Cleaved PARP (Asp214) Antibody (Rat Specific) #9545
PhosphoSitePlus® protein, site, and accession data: PARP1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | R | Endogenous | 89 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9545:
- Western Blotting
Specificity / Sensitivity
Cleaved PARP (Asp214) Antibody (Rat Specific) detects endogenous levels of the large fragment of rat PARP1 (89 kDa) resulting from caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues surrounding Asp214 in rat PARP. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of C6 cells, untreated or staurosporine-treated (1 µM, 3hrs), using Cleaved PARP (Asp214) Antibody (Rat Specific).
Background
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)
- Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
- Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
- Cohen, G.M. (1997) Biochem. J. 326, 1-16.
- Nicholson, D. W. et al. (1995) Nature 376, 37-43.
- Tewari, M. et al. (1995) Cell 81, 801-809.
- Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.
Application References
- Erhardt, J. A. et al. (2001) Activation of caspase-3/caspase-3-like activity in rat cardiomyocytes by an RGD peptide, but not the GP IIb/IIIa antagonist lotrafiban. Thromb. Res. 103, 143-148. Applications: Western Blotting
- Han, H. et al. (2001) Oxidative preconditioning and apoptosis in L-cells. J. Biol. Chem. 276 (28), 26357-26364. Applications: Western Blotting
- Au-Yeung, K. W. et al. (2001) Inhibition of stress-avtivated protein kinase in the ischemic/reperfused heart: role of magnesiuim tanshinoate B in preventing apoptosis. Bio. Pharmacol. 62 (4), 483-493. Applications: Western Blotting
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Companion Products
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9541 Cleaved PARP (Asp214) Antibody (Human Specific)
- 9542 PARP Antibody
- 9544 Cleaved PARP (Asp214) Antibody (Mouse Specific)
- 9803 Cell Lysis Buffer (10X)
For Research Use Only. Not For Use In Diagnostic Procedures.
