Product Pathways - Apoptosis / Autophagy
Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific) #9546
PhosphoSitePlus® protein, site, and accession data: PARP1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H | Endogenous | 89 | Mouse IgG1 |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific) detects endogenous levels of the large fragment (89 kDa) of human PARP1 resulting from cleavage at aspartic acid 214. This antibody will detect high levels of full length PARP.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic PARP peptide corresponding to carboxy-terminal residues surrounding Asp214 in human PARP.
Western Blotting
Western blot analysis of extracts from Jurkat cells, untreated or etoposide-treated (25 µM, 5hrs), using Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific).
Background
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)
- Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
- Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
- Cohen, G.M. (1997) Biochem. J. 326, 1-16.
- Nicholson, D. W. et al. (1995) Nature 376, 37-43.
- Tewari, M. et al. (1995) Cell 81, 801-809.
- Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.
Application References
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Companion Products
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
