Product Pathways - Apoptosis / Autophagy
Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific) #9546
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IF-IC IC | H | Endogenous | 89 | Mouse IgG1 |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
IC=Immunocytochemistry
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific) detects endogenous levels of the large fragment (89 kDa) of human PARP1 resulting from cleavage at aspartic acid 214. The antibody does not recognize full length PARP1 or other PARP isoforms.
Source / Purification
Monoclonal antibody is produced by immunizing mice with a synthetic PARP peptide (KLH coupled) corresponding to carboxy-terminal residues surrounding Asp214 in human PARP.
Western Blotting
Western blot analysis of extracts from Jurkat cells, untreated or etoposide-treated (25 µM), using Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific).
IC-ABC
Immunocytochemical analysis of HeLa cells, untreated or staurosporine-treated, using Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific).
IF-IC
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Confocal immunofluorescent images of HT-29 cells, untreated (left) or Staurosporine #9953 treated (right) labeled with Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific) (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)
- Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
- Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
- Cohen, G.M. (1997) Biochem. J. 326, 1-16.
- Nicholson, D. W. et al. (1995) Nature 376, 37-43.
- Tewari, M. et al. (1995) Cell 81, 801-809.
- Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
