Product Pathways - Apoptosis / Autophagy
Cleaved PARP (Asp214) Antibody (Human Specific) (Fluorescein Conjugate) #9547
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| IC | H | Endogenous | 89 | Rabbit |
Applications Key:
IC=Immunocytochemistry
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Cleaved PARP (Asp214) Antibody (Human Specific) (Fluorescein Conjugate) detects endogenous levels of the large fragment of PARP1 (89 kDa) resulting from cleavage at aspartic acid 214. It is a useful marker for detecting apoptotic cells by immunofluorescence. For fluorescence detection use excitation 494, emission 520 (FITC filter set compatible).
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH coupled) corresponding to carboxy-terminal residues surrounding Asp214 in human PARP. Antibodies are purified by protein A and peptide affinity chromatography.
IF-IC
Immunofluorescent analysis of HeLa cells, untreated (A, C) or staurosporine-treated (B, D), using Cleaved PARP (Asp214) Antibody (Human Specific) (Fluorescein Conjugate). A and B are phase images; C and D are fluorescent images.
Background
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)
- Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
- Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
- Cohen, G.M. (1997) Biochem. J. 326, 1-16.
- Nicholson, D. W. et al. (1995) Nature 376, 37-43.
- Tewari, M. et al. (1995) Cell 81, 801-809.
- Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.
Application References
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Companion Products
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
