Product Pathways - Apoptosis
Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific) #9548
PhosphoSitePlus® protein, site, and accession data: PARP1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | M | Endogenous | 89 | Mouse IgG2b |
Applications Key:
W=Western Blotting
Reactivity Key:
M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9548:
- Western Blotting
Specificity / Sensitivity
Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific) detects endogenous levels of the large fragment of mouse PARP1 (89 kDa) resulting from caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic PARP peptide corresponding to carboxy-terminal residues surrounding Asp214 in mouse PARP.
Western Blotting
Western blot analysis of NIH/3T3 cells, untreated or staurosporine-treated (1 µM), using Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific).
Background
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)
- Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
- Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
- Cohen, G.M. (1997) Biochem. J. 326, 1-16.
- Nicholson, D. W. et al. (1995) Nature 376, 37-43.
- Tewari, M. et al. (1995) Cell 81, 801-809.
- Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.
Application References
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Companion Products
- 9541 Cleaved PARP (Asp214) Antibody (Human Specific)
- 9542 PARP Antibody
- 9544 Cleaved PARP (Asp214) Antibody (Mouse Specific)
- 9545 Cleaved PARP (Asp214) Antibody (Rat Specific)
- 9546 Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific)
- 9803 Cell Lysis Buffer (10X)
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
