Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific) #9548

Applications Reactivity MW (kDa) Source Isotype
W IF-IC M 89 Mouse IgG2b

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  M=Mouse
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific) detects endogenous levels of the large fragment of mouse PARP1 (89 kDa) resulting from caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms.

Source / Purification

Monoclonal antibody is produced by immunizing mice with a synthetic PARP peptide (KLH coupled) corresponding to carboxy-terminal residues surrounding Asp214 in mouse PARP.

Western Blotting

Western Blotting

Western blot analysis of NIH/3T3 cells, untreated or staurosporine-treated (1 µM), using Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific).

IF-IC

IF-IC

Immunofluorescent analysis of NIH/3T3 cells, untreated (left) or staurosporine-treated (right), using Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific).

Background

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)

  1. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
  2. Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
  3. Cohen, G.M. (1997) Biochem. J. 326, 1-16.
  4. Nicholson, D. W. et al. (1995) Nature 376, 37-43.
  5. Tewari, M. et al. (1995) Cell 81, 801-809.
  6. Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.

Application References

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