Product Pathways - Development
Phospho-β-Catenin (Ser33/37/Thr41) Antibody #9561
PhosphoSitePlus® protein, site, and accession data: CTNNB1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk (C) (X) (Z) (B) (Dg) (Pg) | Endogenous | 92 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
C=Chicken
X=Xenopus
Z=Zebrafish
B=Bovine
Dg=Dog
Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9561:
- Western Blotting
Specificity / Sensitivity
Phospho-β-Catenin (Ser33/37/Thr41) Antibody detects endogenous levels of β-catenin only when phosphorylated at serines 33, 37 or threonine 41. It does not recognize beta-catenin phosphorylated at other sites.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser33, Ser37 and Thr41 of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293 cells, pretreated with 20 mM LiCl for 30 minutes and then with 50 nM calyculin A, using Phospho-β-Catenin (Ser33/37/Thr41) Antibody (upper) or β-Catenin Antibody #9562 (lower).
Western Blotting
Western blot analysis of extracts from SW480 cells using Phospho-β-Catenin (Ser33/37/Thr41) Antibody.
Background
β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
- Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
- Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell. Dev. Biol. 14, 59-88.
- Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
- Amit, S. et al. (2002) Genes Dev. 16, 1066-1076.
- Lin, C. et al. (2002) Cell 108, 837-847.
- Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742.
- Yost, C. et al. (1996) Genes Dev. 10, 1443-1454.
- Morin, P.J. (1997) Science 275, 1787-1790.
Application References
- Amit, S. et al. (2002) Axin-mediated CKI phosphorylation of Beta-catenin at Ser 45: a molecular switch for the Wnt pathway. Genes and Development 16, 1066-1076. Applications: Western Blotting
- Hagen, T. et al. (2002) Expression and characterization of GSK-3 mutants and their effect on beta-catenin phosphorylation in intact cells. J. Biol. Chem. 277 (26), 23330-23335. Applications: Western Blotting
- Callow, M.G. et al. (2011) PLoS One 6, e22595. Applications: Western Blotting
- Bone, H.K. et al. (2011) J Cell Sci 124, 1992-2000. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9562 β-Catenin Antibody
- 9564 Phospho-β-Catenin (Ser45) Antibody
- 9565 Phospho-β-Catenin (Thr41/Ser45) Antibody
- 9581 β-Catenin Antibody (Amino-terminal Antigen)
- 9582 β-Catenin (6B3) Rabbit mAb
- 9587 β-Catenin Antibody (Carboxy-terminal Antigen)
- 9534 Acetyl-β-Catenin (Lys49) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
