Product Pathways - Development
Phospho-β-Catenin (Ser33/37/Thr41) Antibody #9561
|W||H M R Mk (C) (X) (Z) (B) (Dg) (Pg)||Endogenous||92||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken X=Xenopus Z=Zebrafish B=Bovine Dg=Dog Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-β-Catenin (Ser33/37/Thr41) Antibody detects endogenous levels of β-catenin only when phosphorylated at serines 33, 37 or threonine 41. It does not recognize beta-catenin phosphorylated at other sites.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser33, Ser37 and Thr41 of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293 cells, pretreated with 20 mM LiCl for 30 minutes and then with 50 nM calyculin A, using Phospho-β-Catenin (Ser33/37/Thr41) Antibody (upper) or β-Catenin Antibody #9562 (lower).
β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
- Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
- Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell. Dev. Biol. 14, 59-88.
- Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
- Amit, S. et al. (2002) Genes Dev. 16, 1066-1076.
- Lin, C. et al. (2002) Cell 108, 837-847.
- Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742.
- Yost, C. et al. (1996) Genes Dev. 10, 1443-1454.
- Morin, P.J. (1997) Science 275, 1787-1790.
- Amit, S. et al. (2002) Axin-mediated CKI phosphorylation of Beta-catenin at Ser 45: a molecular switch for the Wnt pathway. Genes and Development 16, 1066-1076. Applications: Western Blotting
- Hagen, T. et al. (2002) Expression and characterization of GSK-3 mutants and their effect on beta-catenin phosphorylation in intact cells. J. Biol. Chem. 277 (26), 23330-23335. Applications: Western Blotting
- Callow, M.G. et al. (2011) PLoS One 6, e22595. Applications: Western Blotting
- Bone, H.K. et al. (2011) J Cell Sci 124, 1992-2000. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.