Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

Phospho-β-Catenin (Ser45) Antibody #9564

Applications Reactivity Sensitivity MW (kDa) Source
W H (M) (R) (Pg) Endogenous 92 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-β-Catenin (Ser45) Antibody detects endogenous levels of beta-catenin only when phosphorylated at serine 45. It does not recognize β-catenin phosphorylated at other sites.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser45 of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293 cells untreated and treated with calyculin A (50 nM for 30 minutes), using Phospho-β-Catenin (upper) and β-Catenin Antibody #9562 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293 cells untreated and treated with 50 nM calyculin A for 30 minutes, using Phospho-β-Catenin (Ser45) Antibody (lanes 1-2) or the same antibody after preincubation with listed peptides (lanes 3-8). (S45-P is phospho-Ser45 peptide; S33/37/T41-P is phospho-Ser33/37/Thr41 peptide; S45-non-P is nonphospho-Ser45 peptide.)

Western Blotting

Western Blotting

Western blot analysis of extracts from SW480 cells, using Phospho-β-Catenin (Ser45) Antibody.


Background

β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
  2. Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell. Dev. Biol. 14, 59-88.
  3. Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev. 16, 1066-1076.
  5. Lin, C. et al. (2002) Cell 108, 837-847.
  6. Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742.
  7. Yost, C. et al. (1996) Genes Dev. 10, 1443-1454.
  8. Morin, P.J. (1997) Science 275, 1787-1790.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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