Product Pathways - Wnt / Hedgehog / Notch
Phospho-β-Catenin (Thr41/Ser45) Antibody #9565
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W IHC-P IC | H M Mk | 92 | Rabbit |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IC=Immunocytochemistry
Reactivity Key:
H=Human
M=Mouse
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-β-Catenin (Thr41/Ser45) Antibody detects endogenous levels of beta-catenin only when phosphorylated at Thr41 or Ser45. This antibody does not recognize β-catenin phosphorylated at other sites.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr41 and Ser45 of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293 cells pretreated with 20 mM LiCl for 30 minutes and then with 50 nM calyculin A, using Phospho-β-Catenin (Thr41/Ser45) Antibody (upper) or β-Catenin Antibody #9562 (lower).
Western Blotting
Western blot analysis of extracts from SW480 cells using Phospho-β-Catenin (Thr41/Ser45) Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Phospho-β-Catenin (Thr41/Ser45) Antibody.
Background
β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin on Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37 and Thr41 (7). Mutations in these phosphorylation sites, which result in the stabilization of β-catenin protein levels, have been found in many tumor cell lines (8).
- Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
- Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell. Dev. Biol. 14, 59-88.
- Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
- Amit, S. et al. (2002) Genes Dev. 16, 1066-1076.
- Lin, C. et al. (2002) Cell 108, 837-847.
- Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742.
- Yost, C. et al. (1996) Genes Dev. 10, 1443-1454.
- Morin, P.J. (1997) Science 275, 1787-1790.
Application References
- Amit, S. et al. (2002) Axin-mediated CKI phosphorylation of Beta-catenin at Ser 45: a molecular switch for the Wnt pathway. Genes and Development 16, 1066-1076. This article references the use of Phospho-β-Catenin (Thr41/Ser45) Antibody in the following applications: Western Blotting
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Companion Products
- 9561 Phospho-β-Catenin (Ser33/37/Thr41) Antibody
- 9562 β-Catenin Antibody
- 1130 Phospho-β-Catenin (Thr41/Ser45) Blocking Peptide
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
