Product Pathways - Development
Phospho-β-Catenin (Thr41/Ser45) Antibody #9565
|9565S||100 µl (10 western blots)||---||In Stock||---|
|9565P||40 µl (4 western blots)||---||In Stock||---|
|9565||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Monkey||Endogenous||92||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Dog, Pig.
Specificity / Sensitivity
Phospho-β-Catenin (Thr41/Ser45) Antibody detects endogenous levels of beta-catenin only when phosphorylated at Thr41 or Ser45. This antibody does not recognize β-catenin phosphorylated at other sites.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr41 and Ser45 of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293 cells pretreated with 20 mM LiCl for 30 minutes and then with 50 nM calyculin A, using Phospho-β-Catenin (Thr41/Ser45) Antibody (upper) or β-Catenin Antibody #9562 (lower).
β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
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