Product Pathways - Akt Signaling
Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb #9570
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP E-P | B (H) (M) (R) | Endogenous | 140 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
E-P=ELISA (Peptide)
Reactivity Key:
H=Human
M=Mouse
R=Rat
B=Bovine
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb detects endogenous levels of eNOS only when phosphorylated at Ser1177.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser1177 of human eNOS.
Western Blotting
Western blot analysis of extracts from BAEC cells, phosphatase or VEGF-treated, using Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb (upper) or eNOS Antibody #9572 (lower).
Background
Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation (6,7).
- Fulton, D. et al. (2001) J. Pharmacol. Exp. Ther. 299, 818-824.
- Shaul, P.W. (2002) Annu. Rev. Physiol. 64, 749-774.
- Chen, Z.P. et al. (1999) FEBS Lett. 443, 285-289.
- Dimmeler, S. et al. (1999) Nature 399, 601-605.
- Fulton, D. et al. (1999) Nature 399, 597-601.
- Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
- Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9575 Phospho-eNOS (Ser113) Antibody
- 9574 Phospho-eNOS (Thr495) Antibody
- 9571 Phospho-eNOS (Ser1177) Antibody
- 9572 eNOS Antibody
- 9586 eNOS (49G3) Rabbit mAb
- 9271 Phospho-Akt (Ser473) Antibody
- 9275 Phospho-Akt (Thr308) Antibody
- 9272 Akt Antibody
- 1133 eNOS (Ser1177) Biotinylated Peptide
- 7501 HTScan® Akt1 Kinase Assay Kit
- 7504 HTScan® Akt2 Kinase Assay Kit
- 7507 HTScan® Akt3 Kinase Assay Kit
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
