Product Pathways - Akt Signaling
Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb #9570
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP E-P | B (H) (M) (R) | Endogenous | 140 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
E-P=ELISA (Peptide)
Reactivity Key:
H=Human
M=Mouse
R=Rat
B=Bovine
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb detects endogenous levels of eNOS only when phosphorylated at Ser1177.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser1177 of human eNOS.
Background
Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation (6,7).
- Fulton, D. et al. (2001) J. Pharmacol. Exp. Ther. 299, 818-824.
- Shaul, P.W. (2002) Annu. Rev. Physiol. 64, 749-774.
- Chen, Z.P. et al. (1999) FEBS Lett. 443, 285-289.
- Dimmeler, S. et al. (1999) Nature 399, 601-605.
- Fulton, D. et al. (1999) Nature 399, 597-601.
- Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
- Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.
Application References
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Companion Products
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.