Product Pathways - PI3K / Akt Signaling
Phospho-eNOS (Ser1177) Antibody #9571
PhosphoSitePlus® protein, site, and accession data: eNOS
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | B Pg (H) (M) (R) | Endogenous | 140 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
B=Bovine
Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9571:
- Western Blotting
Specificity / Sensitivity
Phospho-eNOS (Ser1177) Antibody detects endogenous levels of eNOS only when phosphorylated at Ser1177.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1177 of human eNOS. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from bovine aortic endothelial cells (BAECs), untreated or VEGF-treated (50 ng/ml) for the indicated times, using Phospho-eNOS (Ser1177) Antibody (upper) or control eNOS antibody (lower).
Background
Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).
- Fulton, D. et al. (2001) J. Pharmacol. Exp. Ther. 299, 818-824.
- Shaul, P.W. (2002) Annu. Rev. Physiol. 64, 749-774.
- Chen, Z.P. et al. (1999) FEBS Lett. 443, 285-289.
- Dimmeler, S. et al. (1999) Nature 399, 601-605.
- Fulton, D. et al. (1999) Nature 399, 597-601.
- Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
- Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.
Application References
- Brouet, A. et al. (2001) J Biol Chem 276, 32663-9. Applications: Western Blotting
- Thomas, S.R. et al. (2002) J Biol Chem 277, 6017-24. Applications: Western Blotting
- Du, X.L. et al. (2001) J Clin Invest 108, 1341-8. Applications: Western Blotting
- Boo, Y.C. et al. (2002) J Biol Chem 277, 3388-96. Applications: Western Blotting
- Makarova, A.M. et al. (2011) J Biol Chem 286, 23044-53. Applications: IC-IF Western Blotting
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Companion Products
- 9570 Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb
- 9572 eNOS Antibody
- 9574 Phospho-eNOS (Thr495) Antibody
- 9586 eNOS (49G3) Rabbit mAb
- 1133 eNOS (Ser1177) Biotinylated Peptide
- 9271 Phospho-Akt (Ser473) Antibody
- 9272 Akt Antibody
- 9275 Phospho-Akt (Thr308) Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
