Product Pathways - Akt Signaling

PTEN (138G6) Rabbit mAb (Alexa Fluor® 647 Conjugate) #9573

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Applications	Reactivity	Source	Isotype
F	H M R Mk	Rabbit	IgG

Applications Key:  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

PTEN (138G6) Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total PTEN protein.

Source / Purification

Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the carboxy-terminal sequence of human PTEN. The antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-5. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.

Description

Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells.*The unconjugated antibody #9559 reacts with human, monkey, mouse and rat PTEN protein. CST expects that PTEN (138G6) Rabbit mAb (Alexa Fluor® 647 Conjugate) will also recognize PTEN in these species.

Background

PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382 and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein level and activity (8) and is involved in G protein coupled signaling during chemotaxis (9,10).

1. Cantley, L.C. and Neel, B.G. (1999) Proc. Natl. Acad. Sci. USA 96, 4240-4245.
2. Myers, M.P. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 9052-9057.
3. Myers, M.P. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 13513-13518.
4. Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-8211.
5. Wu, X. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15587-15591.
6. Vazquez, F. et al. (2000) Mol. Cell. Biol. 20, 5010-5018.
7. Torres, J. and Pulido, R. (2001) J. Biol. Chem. 276, 993-998.
8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-130.
9. Funamoto, S. et al. (2002) Cell 109, 611-623.
10. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.

Application References

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Companion Products

• 1250 PTEN Blocking Peptide
• 2336 Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor^® 488 Conjugate)

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.