Product Pathways - PI3K / Akt Signaling
Phospho-eNOS (Thr495) Antibody #9574
|9574S||100 µl (10 western blots)||---||In Stock||---|
|9574||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Bovine, Pig||Endogenous||140||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Mouse, Rat.
Specificity / Sensitivity
Phospho-eNOS (Thr495) Antibody detects endogenous levels of eNOS only when phosphorylated at threonine 495.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr495 of human eNOS. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from porcine aortic endothelial cells (PAECs), 3 days post-confluence, untreated, H2O2-treated (0.3 mM for 30 minutes) or calf intestinal alkaline phosphatase (CIP)-treated as indicated, using Phospho-eNOS (Thr495) Antibody (upper) or control eNOS antibody (lower).
Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).
- Fulton, D. et al. (2001) J. Pharmacol. Exp. Ther. 299, 818-824.
- Shaul, P.W. (2002) Annu. Rev. Physiol. 64, 749-774.
- Chen, Z.P. et al. (1999) FEBS Lett. 443, 285-289.
- Dimmeler, S. et al. (1999) Nature 399, 601-605.
- Fulton, D. et al. (1999) Nature 399, 597-601.
- Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
- Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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