Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-eNOS (Ser113) Antibody #9575

Applications Reactivity Sensitivity MW (kDa) Source
W H B Endogenous 140 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-eNOS (Ser113) Antibody detects endogenous levels of eNOS only when phosphorylated at serine 113.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser113 of human eNOS. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from bovine aoric endothelial cells (BAEC), untreated or treated with lamda phosphatase, using Phospho-eNOS (Ser113) Antibody (upper) or eNOS antibody (lower).

Background

Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).

eNOS is activated by VEGF, and this activation is associated with dephsophorylation of eNOS at serine 113. Cyclosporin A blocks this dephosphorylation of eNOS upon VEGF stimulation (8).

  1. Fulton, D. et al. (2001) J. Pharmacol. Exp. Ther. 299, 818-824.
  2. Shaul, P.W. (2002) Annu. Rev. Physiol. 64, 749-774.
  3. Chen, Z.P. et al. (1999) FEBS Lett. 443, 285-289.
  4. Dimmeler, S. et al. (1999) Nature 399, 601-605.
  5. Fulton, D. et al. (1999) Nature 399, 597-601.
  6. Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
  7. Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.
  8. Kou, R. et al. (2002) J Biol. Chem. 277(33) , 29669-29673.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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