Product Pathways - Apoptosis
Cleaved Drosophila Dcp-1 (Asp216) Antibody #9578
Reactivity Key: Dm=D. melanogaster
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Cleaved Drosophila Dcp-1 (Asp216) Antibody recognizes endogenous levels of the large 22 kDa fragment of cleaved Dcp-1. This antibody does not recognize full length Dcp-1. The antibody also detects a non-specific, apoptotic-related band at 50 kDa by western blot.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp216 of Drosophila Dcp-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from Drosophila S2 cells, untreated (-) or treated with either cycloheximide (10 μM, 5 hr; +) or actinomycin D (0.7 μM, 5 hr; +), using Cleaved Drosophila Dcp-1 (Asp216) Antibody.
Confocal immunofluorescent analysis of S2 cells, untreated (left) or treated with Actinomycin D (0.7 uM, 20 hr; right), using Cleaved Drosophila Dcp-1 (Asp216) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of wild-type (white1118) Drosophila egg chambers, conditioned (left) or conditioned followed by starvation (right), using Cleaved Drosophila Dcp-1 (Asp216) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Cell death in the fruit fly Drosophila melanogaster is regulated by many of the same stimuli as mammalian cell death (1). The Drosophila genome contains seven caspase genes; three encode initiator caspases, and four encode effector caspases (reviewed in (2)). The Drosophila effector caspase, death caspase-1 (Dcp-1), is a critical executioner of apoptosis. It is involved in the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). The activation of Dcp-1 requires proteolytic processing of its inactive zymogen into active p22 and p13 fragments (3). Comparison of the in vivo activity between DrICE and Dcp-1 has shown that DrICE is a more effective inducer of apoptosis than Dcp-1, which instead plays a role in determining the rate of cell death (4).
- Steller, H. et al. (1994) Philos Trans R Soc Lond B Biol Sci 345, 247-50.
- Hay, B.A. and Guo, M. (2006) Annu Rev Cell Dev Biol 22, 623-50.
- Song, Z. et al. (1997) Science 275, 536-40.
- Florentin, A. and Arama, E. (2012) J Cell Biol 196, 513-27.
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For Research Use Only. Not For Use In Diagnostic Procedures.