Product Pathways - Apoptosis
Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb #9579
PhosphoSitePlus® protein, site, and accession data: Casp3
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IHC-P IF-IC F | H (M) (R) (Mk) (B) (Pg) | Endogenous | Rabbit IgG |
Applications Key:
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9579:
- Flow, IHC / Paraffin, Immunofluorescence
Specificity / Sensitivity
Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb recognizes endogenous levels of caspase-3 protein only when cleaved at Asp175. This antibody is preferred for immunofluorescence, flow cytometry, and immunohistochemistry applications. This antibody detects non-specific caspase substrates by western blot.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp175 of human caspase-3 protein.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded Jurkat cell pellets, untreated (left) or etoposide-treated (right), using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb on SignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls #8104.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HT-29 xenograft using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb in the presence of control peptide (left) or Cleaved Caspase-3 (Asp175) Blocking Peptide #1050 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human malignant stromal tumor using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Staurosporine #9953 (1 μM, 4 hr; right), using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).
- Fernandes-Alnemri, T. et al. (1994) J. Biol. Chem. 269, 30761-30764.
- Nicholson, D. W. et al. (1995) Nature 376, 37-43.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9664 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb
- 9661 Cleaved Caspase-3 (Asp175) Antibody
- 9665 Caspase-3 (8G10) Rabbit mAb
- 9668 Caspase-3 (3G2) Mouse mAb
- 1050 Cleaved Caspase-3 (Asp175) Blocking Peptide
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 8104 SignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls
- 5425 Normal Goat Serum
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
For Research Use Only. Not For Use In Diagnostic Procedures.
