Product Pathways - Wnt / Hedgehog / Notch
β-Catenin Antibody (Amino-terminal Antigen) #9581
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-F F ChIP | H M R Mk Hm B | Endogenous | 92 kDa | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-F=Immunofluorescence (Frozen)
F=Flow Cytometry
ChIP=Chromatin IP
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Hm=Hamster
B=Bovine
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
Beta-Catenin Antibody detects endogenous levels of total β-catenin protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp56 of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of total cell extracts from 293 and NIH/3T3 cells, using β-Catenin Antibody (Amino-terminal Antigen).
Flow Cytometry
Flow cytometric analysis of 293 cells, using β-Catenin Antibody (Amino-terminal Antigen) (blue) compared to a nonspecific negative control antibody (red).
IF-F
Confocal immunofluorescence image of mouse colon labeled with β-Catenin Antibody (Amino-terminal Antigen) (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT116 cells and either 20 μl of β-Catenin Antibody (Amino-terminal Antigen) #9581 or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP™ Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by Real-Time PCR using primers specific for the CAMK2D and c-MYC genes, and the heterochromatic Alpha Satellite repeat element. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Background
β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin on Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37 and Thr41 (7). Mutations in these phosphorylation sites, which result in the stabilization of β-catenin protein levels, have been found in many tumor cell lines (8).
- Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
- Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell. Dev. Biol. 14, 59-88.
- Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
- Amit, S. et al. (2002) Genes Dev. 16, 1066-1076.
- Lin, C. et al. (2002) Cell 108, 837-847.
- Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742.
- Yost, C. et al. (1996) Genes Dev. 10, 1443-1454.
- Morin, P.J. (1997) Science 275, 1787-1790.
Application References
- Yochum, G.S. et al. (2007) Proc Natl Acad Sci U S A 104, 3324-9. Applications: ChIP
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9561 Phospho-β-Catenin (Ser33/37/Thr41) Antibody
- 9565 Phospho-β-Catenin (Thr41/Ser45) Antibody
- 9587 β-Catenin Antibody (Carboxy-terminal Antigen)
- 3216 Dvl2 Antibody
- 3218 Dvl3 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
