Product Pathways - Wnt / Hedgehog / Notch
β-Catenin (6B3) Rabbit mAb #9582
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP IHC-P IHC-F | H M R Mk | 92 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IHC-F=Immunohistochemistry (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
β-Catenin (6B3) Rabbit mAb detects endogenous levels of total β-catenin.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (Blue Carrier-coupled) corresponding to the carboxy terminus of human β-catenin.
Western Blotting
Western blot analysis of total cell lysates from 293, Rat2 and COS cells, using β-Catenin (6B3) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using β-Catenin (6B3) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using β-Catenin (6B3) Rabbit mAb.
IHC-F (frozen)
Immunohistochemical analysis of frozen HCC-827 xenograft using beta-Catenin (6B3) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of untreated HeLa cells, using β-Catenin (6B3) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
Background
β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin on Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37 and Thr41 (7). Mutations in these phosphorylation sites, which result in the stabilization of β-catenin protein levels, have been found in many tumor cell lines (8).
- Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
- Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell. Dev. Biol. 14, 59-88.
- Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
- Amit, S. et al. (2002) Genes Dev. 16, 1066-1076.
- Lin, C. et al. (2002) Cell 108, 837-847.
- Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742.
- Yost, C. et al. (1996) Genes Dev. 10, 1443-1454.
- Morin, P.J. (1997) Science 275, 1787-1790.
Application References
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Companion Products
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
