Product Pathways - Akt Signaling
eNOS (49G3) Rabbit mAb #9586
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H B (M) (R) | Endogenous | 140 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
B=Bovine
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
eNOS (49G3) Rabbit mAb detects endogenous levels of total eNOS protein.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human eNOS.
Background
Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation (6,7).
- Fulton, D. et al. (2001) J. Pharmacol. Exp. Ther. 299, 818-824.
- Shaul, P.W. (2002) Annu. Rev. Physiol. 64, 749-774.
- Chen, Z.P. et al. (1999) FEBS Lett. 443, 285-289.
- Dimmeler, S. et al. (1999) Nature 399, 601-605.
- Fulton, D. et al. (1999) Nature 399, 597-601.
- Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
- Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.
Application References
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Companion Products
- 9575 Phospho-eNOS (Ser113) Antibody
- 9271 Phospho-Akt (Ser473) Antibody
- 9574 Phospho-eNOS (Thr495) Antibody
- 9571 Phospho-eNOS (Ser1177) Antibody
- 9572 eNOS Antibody
- 9275 Phospho-Akt (Thr308) Antibody
- 9272 Akt Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.