Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

eNOS (49G3) Rabbit mAb #9586

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H B (M) (R) Endogenous 140 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

eNOS (49G3) Rabbit mAb detects endogenous levels of total eNOS protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human eNOS.

Western Blotting

Western Blotting

Western blot analysis of extracts from BAEC and HUVEC cells, using eNOS (49G3) Rabbit mAb.

Background

Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).

  1. Fulton, D. et al. (2001) J. Pharmacol. Exp. Ther. 299, 818-824.
  2. Shaul, P.W. (2002) Annu. Rev. Physiol. 64, 749-774.
  3. Chen, Z.P. et al. (1999) FEBS Lett. 443, 285-289.
  4. Dimmeler, S. et al. (1999) Nature 399, 601-605.
  5. Fulton, D. et al. (1999) Nature 399, 597-601.
  6. Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
  7. Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.


For Research Use Only. Not For Use In Diagnostic Procedures.

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