Product Pathways - Wnt / Hedgehog / Notch
Slug (L40C6) Mouse mAb #9589
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H | Endogenous | 30 | Mouse IgG2a kappa |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Slug (L40C6) Mouse mAb detects endogenous levels of total Slug protein. It does not recognize transfected human or mouse Snail proteins.
Source / Purification
Monoclonal antibody is produced by immunizing mice with a recombinant human Slug protein.
Background
Slug (SNAI2) is a widely expressed transcriptional repressor and member of the Snail family of zinc finger transcription factors (1). Similar to the related Snail protein, Slug binds to the E-cadherin promoter region to repress transcription during development (2). The binding of Slug to integrin promoter sequences represses integrin expression and results in reduced cell adhesion (3). Down regulation of E-cadherin expression occurs during the epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (4,5). The tumor suppressor protein p53 induces Slug expression in γ-irradiated cells; Slug protects damaged cells from apoptosis by repressing p53-induced transcription of the proapoptotic Bcl-2 family protein Puma (6). Deletion mutations in the corresponding Slug gene are associated with the pigmentation disorders Waardenburg Syndrome and Piebaldism, while a genetic duplication resulting in Slug overexpression is associated with a collection of congenital heart defects termed tetralogy of Fallot (7).
- Inukai, T. et al. (1999) Mol Cell 4, 343-52.
- Bolos, V. et al. (2003) J Cell Sci 116, 499-511.
- Turner, F.E. et al. (2006) J Biol Chem 281, 21321-31.
- Barrallo-Gimeno, A. and Nieto, M.A. (2005) Development 132, 3151-61.
- Castro Alves, C. et al. (2007) J Pathol 211, 507-15.
- Wu, W.S. et al. (2005) Cell 123, 641-53.
- Perez-Mancera, P.A. et al. (2006) Cytogenet Genome Res 114, 24-9.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.