Cell Signaling Technology

Product Pathways - Development

Slug (L40C6) Mouse mAb #9589

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 30 Mouse IgG2a

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Slug (L40C6) Mouse mAb detects endogenous levels of total Slug protein. It does not recognize transfected human or mouse Snail proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human Slug protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from A204 and SK-MEL-5 cells using Slug (L40C6) Mouse mAb.

Background

Slug (SNAI2) is a widely expressed transcriptional repressor and member of the Snail family of zinc finger transcription factors (1). Similar to the related Snail protein, Slug binds to the E-cadherin promoter region to repress transcription during development (2). The binding of Slug to integrin promoter sequences represses integrin expression and results in reduced cell adhesion (3). Down regulation of E-cadherin expression occurs during the epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (4,5). The tumor suppressor protein p53 induces Slug expression in γ-irradiated cells; Slug protects damaged cells from apoptosis by repressing p53-induced transcription of the proapoptotic Bcl-2 family protein Puma (6). Deletion mutations in the corresponding Slug gene are associated with the pigmentation disorders Waardenburg Syndrome and Piebaldism, while a genetic duplication resulting in Slug overexpression is associated with a collection of congenital heart defects termed tetralogy of Fallot (7).

  1. Inukai, T. et al. (1999) Mol Cell 4, 343-52.
  2. Bolós, V. et al. (2003) J Cell Sci 116, 499-511.
  3. Turner, F.E. et al. (2006) J Biol Chem 281, 21321-31.
  4. Barrallo-Gimeno, A. and Nieto, M.A. (2005) Development 132, 3151-61.
  5. Castro Alves, C. et al. (2007) J Pathol 211, 507-15.
  6. Wu, W.S. et al. (2005) Cell 123, 641-53.
  7. Pérez-Mancera, P.A. et al. (2006) Cytogenet Genome Res 114, 24-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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