Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

SMG-1 (V72) Antibody #9592

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC H Mk Endogenous 410 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SMG-1 (V72) Antibody detects endogenous levels of total SMG-1 protein. The antibody cross-reacts with a 100 kDa band of unknown origin. By confocal immunofluorescence, the antibody labels the mitochondria of U-2 OS cells (7).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SMG-1.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29, COS and U-2 OS cells using SMG-1 (V72) Antibody.

Background

SMG-1 is a member of the phosphoinositide 3-kinase-related kinase (PIKK) family, which includes ATM, ATR, mTOR, DNA-PKcs, and TRRAP (1,2). Activated by DNA damage, SMG-1 has been shown to phosphorylate p53 and hUpf1 (SMG-2) (1-4). hUpf1 is a subunit of the surveillance complex that allows degradation of messenger RNA species containing premature termination codons (PTCs). This process, known as nonsense-mediated mRNA decay (NMD), prevents the translation of truncated forms of proteins that may result in gain of function or dominant negative species. NMD occurs under normal cellular conditions as well as in response to damage (5,6). SMG-1 has also been shown to affect cell death receptor signaling and to protect cells from extrinsically induced apoptotic cell death (7).

  1. Denning, G. et al. (2001) J Biol Chem 276, 22709-14.
  2. Yamashita, A. et al. (2001) Genes Dev 15, 2215-28.
  3. Brumbaugh, K.M. et al. (2004) Mol Cell 14, 585-98.
  4. Ohnishi, T. et al. (2003) Mol Cell 12, 1187-200.
  5. Li, Z.Y. et al. (2006) Curr Med Chem 13, 1693-705.
  6. Mendell, J.T. et al. (2004) Nat Genet 36, 1073-8.
  7. Oliveira, V. et al. (2008) J Biol Chem 283, 13174-84.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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