Product Pathways - Cell Cycle / Checkpoint
SMG-1 (V72) Antibody #9592
|9592S||100 µl (10 western blots)||---||In Stock||---|
|9592||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
SMG-1 (V72) Antibody detects endogenous levels of total SMG-1 protein. The antibody cross-reacts with a 100 kDa band of unknown origin. By confocal immunofluorescence, the antibody labels the mitochondria of U-2 OS cells (7).
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SMG-1.
Western blot analysis of extracts from HT-29, COS and U-2 OS cells using SMG-1 (V72) Antibody.
SMG-1 is a member of the phosphoinositide 3-kinase-related kinase (PIKK) family, which includes ATM, ATR, mTOR, DNA-PKcs, and TRRAP (1,2). Activated by DNA damage, SMG-1 has been shown to phosphorylate p53 and hUpf1 (SMG-2) (1-4). hUpf1 is a subunit of the surveillance complex that allows degradation of messenger RNA species containing premature termination codons (PTCs). This process, known as nonsense-mediated mRNA decay (NMD), prevents the translation of truncated forms of proteins that may result in gain of function or dominant negative species. NMD occurs under normal cellular conditions as well as in response to damage (5,6). SMG-1 has also been shown to affect cell death receptor signaling and to protect cells from extrinsically induced apoptotic cell death (7).
- Denning, G. et al. (2001) J Biol Chem 276, 22709-14.
- Yamashita, A. et al. (2001) Genes Dev 15, 2215-28.
- Brumbaugh, K.M. et al. (2004) Mol Cell 14, 585-98.
- Ohnishi, T. et al. (2003) Mol Cell 12, 1187-200.
- Li, Z.Y. et al. (2006) Curr Med Chem 13, 1693-705.
- Mendell, J.T. et al. (2004) Nat Genet 36, 1073-8.
- Oliveira, V. et al. (2008) J Biol Chem 283, 13174-84.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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