Cell Signaling Technology

Product Pathways - Adhesion

Lyric/Metadherin (2F11C3) Mouse mAb #9596

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Mk Endogenous 65, 75 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Lyric/Metadherin (2F11C3) Mouse mAb detects endogenous levels of total lyric/metadherin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human lyric/metadherin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissue using Lyric/Metadherin (2F11C3) Mouse mAb.

Background

Lyric/AEG-1 (Astrocyte Elevated Gene 1)/MTDH (Metadherin) was identified as a tight junction (TJ) protein based on its localization to TJ proteins in polarized epithelium (1).Differential subcellular localization and overexpression of Lyric/AEG-1/MTDH has been seen in multiple human cancers. Lyric/AEG-1/MTDH is involved in signaling pathways related to various cellular functions including proliferation and apoptosis/survival, and its alteration in cancer is associated with poor prognosis (reviewed in 2). In breast cancer, increased Lyric/AEG-1/MTDH may confer increased chemoresistance as well as metastasis (3,4). Lyric/AEG-1/MTDH expression is important in signaling and disease progression of hepatocellular carcinoma (HCC) (5) and glioblastoma multiforme (GBM) (6).

  1. Britt, D.E. et al. (2004) Exp Cell Res 300, 134-48.
  2. Hu, G. et al. (2009) Clin Cancer Res 15, 5615-20.
  3. Brown, D.M. and Ruoslahti, E. (2004) Cancer Cell 5, 365-74.
  4. Hu, G. et al. (2009) Cancer Cell 15, 9-20.
  5. Yoo, B.K. et al. (2009) J Clin Invest 119, 465-77.
  6. Emdad, L. et al. (2010) Mol Cancer Ther 9, 79-88.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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