Cell Signaling Technology

Product Pathways - Metabolism

MAP4K3 Antibody #9613

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 101 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MAP4K3 Antibody recognizes endogenous levels of total MAP4K3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly407 of human MAP4K3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MAP4K3 Antibody.

Background

The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by growth factors and nutrients including amino acids (1,2). MAP4K3 is a mediator between nutrient signal and mTORC1 (1). Research studies suggest that amino acid sufficiency leads to the phosphorylation of Ser170 on MAP4K3, which activates mTORC1 (3).

  1. Findlay, G.M. et al. (2007) Biochem J 403, 13-20.
  2. Sancak, Y. et al. (2008) Science 320, 1496-501.
  3. Yan, L. et al. (2010) Mol Cell 37, 633-42.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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