Product Pathways - Motif Antibodies

Phospho-(Ser) Kinase Substrate Antibody Sampler Kit #9615

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Kit Includes	Quantity	Applications	Reactivity	MW (kDa)	Source
Phospho-(Ser) CDKs Substrate Antibody # 2324	40 microliters	W IP IHC-P E-P	All		Rabbit
Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb # 2325	40 microliters	W IP IHC-P E-P	All		Rabbit
Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody # 2981	40 microliters	W IP E-P	All		Rabbit
Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb # 9606	40 microliters	W IP E-P	All		Mouse
Anti-rabbit IgG, HRP-linked Antibody # 7074	100 microliters				Goat
Anti-mouse IgG, HRP-linked Antibody # 7076	50 microliters				Horse

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  E-P=ELISA (Peptide)
Reactivity Key:  H=Human  M=Mouse  All=All

Specificity / Sensitivity

Each antibody detects endogenous levels of phospho-(Ser) proteins of specific kinase substrate groups. For more detailed information please see datasheet for this product.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with phospho-peptides containing the kinase substrate motif and purified by protein A and peptide affinity chromatography.Rabbit monoclonal antibody is produced by immunizing rabbits with synthetic phospho-MAPK/CDK substrate peptides.Mouse monoclonal antibody is produced by immunizing mice with phospho-(Ser) 14-3-3 binding motif peptides (KLH-coupled).

Background

Phospho-(Ser) kinases and phosphatases play critical roles in a wide range of biological processes. Each phospho-(Ser) kinase phosphorylates serine within a specific motif. The MAPK and CDK families of serine protein kinases phosphorylate serine followed by proline residue (1-3). The consensus amino acid sequence for CDK substrate is (K/R)(S*)PX(K/R), where X denotes any one of the 20 amino acids and S* is the phosphorylation site (4-6). MAPK phosphorylates substrates with the concensus sequence PX(S*)P. The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control (7-11). Binding of 14-3-3 is mediated through phospho-serine-containing proteins (12). Two different phospho-serine containing motifs are found using a degenerate phospho-serine-oriented peptide library technique, RSXS*XP and RXY/FXS*XP (12). Motif 1 (Arg/Lys and Ser at positions -3 and -2, phospho-Ser at position 0, and Pro at position +2) is found in critical regulatory proteins including Bad, cdc25C, FoxO3A, PKC and c-Raf (11, 13). Motif 2 (RXY/FXS*XP) is found in critical regulatory proteins including cdc25A, cdc25B, PKCγ, IRS-1 and BCR (12). Although Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody binds 14-3-3 binding motif 2 with no requirement for proline in the +2 position, it provides a powerful tool for the discovery and characterization of potential 14-3-3 binding motif 2-containing proteins or other proteins with the RXY/FXS* motif. Antibodies specific to particular kinase substrates are invaluable reagents in determining kinase activity and identifying potential new kinase substrates. CST has developed antibodies that recognize phosphorylated serine within the context of a protein motif that is phosphorylated by MAPK/CDK, CDKs or 14-3-3. As shown by DELFIA or ELISA, each phospho-(Ser) kinase substrate antibody in this sampler kit is specific to its kinase substrate motif.

1. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
2. Karin, M. (1994) Curr Opin Cell Biol 6, 415-24.
3. Lewis, T.S. et al. (1998) Adv Cancer Res 74, 49-139.
4. Songyang, Z. et al. (1996) Mol Cell Biol 16, 6486-93.
5. Songyang, Z. (1999) Prog Biophys Mol Biol 71, 359-72.
6. Holmes, J.K. and Solomon, M.J. (1996) J Biol Chem 271, 25240-6.
7. Aitken, A. (1995) Trends Biochem Sci 20, 95-7.
8. Zha, J. et al. (1996) Cell 87, 619-28.
9. Piwnica-Worms, H. (1999) Nature 401, 535, 537.
10. Tzivion, G. et al. (1998) Nature 394, 88-92.
11. Xing, H. et al. (2000) EMBO J 19, 349-58.
12. Muslin, A.J. et al. (1996) Cell 84, 889-97.
13. Yaffe, M.B. et al. (1997) Cell 91, 961-71.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 9920 Phospho-(Ser/Thr) Kinase Substrate Antibody Sampler Kit
• 9601 Phospho-(Ser) 14-3-3 Binding Motif Antibody
• 9614 Phospho-Akt Substrate (RXRXXS/T) (110B7E) Rabbit mAb
• 2851 Phospho-(Ser/Thr) ATM/ATR Substrate Antibody
• 9441 Acetylated-Lysine Antibody
• 2321 Phospho-(Thr) MAPK/CDK Substrate Mouse mAb
• 4391 Phospho-MAPK Substrates (PXTP) (46G11) Rabbit mAb
• 3821 Phospho-(Tyr) p85 PI3K Binding Motif Antibody
• 9634 Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb
• 9624 Phospho-PKA Substrate (RRXS/T) (100G7E) Rabbit mAb
• 9631 Phospho-(Ser/Thr) Phe Antibody
• 9381 Phospho-Threonine Antibody (P-Thr-Polyclonal)
• 9391 Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)
• 9691 Nitro-Tyrosine Antibody
• 9411 Phospho-Tyrosine Mouse mAb (P-Tyr-100)

License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commerical licensing terms please contact CST Business Development at cbunker@cellsignal.com.